2D-LC User Guide 143
6 Method Parameters
Set the 2D-LC Method parameters
Specify the Switch Time of the Diverter Valve
The diverter valve can be used to automatically divert salt or buffers coming from
the
1
D mobile phase to waste at the beginning of every
2
D analysis.
This section is active only if a diverter valve is included in the 2D-LC Cluster
configuration.
1 To turn on switching of the diverter valve, mark the check box Use Diverter
Valve.
The Switch time field becomes active.
2 Specify a switch time in the Switch time field.
The valve is switched to the detector at the specified time after the start of the
2
D analysis and switched back to waste when the
2
D analysis has finished.
Set up the Gradient Time Table for the Analytical Gradient
Use this section to set up the eluent gradient timetable for the
2
D analysis.
1 Specify the time for the change of solvent composition in the Time[min] field.
2 Specify the percentage of solvent that channel B delivers at the specified time
in the B[%] field.
Channel A always delivers the remaining volume, %A = (100 - %B). The solvent
composition changes linearly from one setpoint to the next.
3 To remove the checkmark in the Shift box, select the single Analytical
Gradient Event first and then clear the corresponding settings in the Gradient
Shift
1
D Time table.
The initial start composition is defined in the
2
D solvents table.
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