Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 67
Using the Pump
3
Setting up the Pump with the Instrument Control Interface
Method Parameter Settings
The pump method setup parameters are in eight sections:
• Flow
•Solvent(s)
•Stoptime
•Posttime
• Pressure Limits
• Timetable
•Advanced
Table 1 2 Method parameters
Parameter Limits Description
Flow ≤200 bar: 0.0 –
10.00 mL/min in
steps of 0.001
> 200 bar: 0.0 –
5.00 mL/min in steps
of 0.001.
The flow is the rate of movement of eluent along the column. It is
important that the flow rate is kept constant to ensure precise retention
time, and peak measurements. Variations in flow rate can occur as a
result of the partial failure of the pumping system, air in the pumping
system, a change in the mobile phase viscosity or a temperature change.
Solvent(s) Isocratic Pump: The text box allows you to type a brief description of the
solvent.
Quaternary Pump: You can set the percentages of solvents B, C and D to
any value from 0 through 100 %. Solvent A always delivers the remaining
volume: 100 - (%B + %C + %D). The check boxes allows you to turn the
solvent channels on (checked) or off (cleared). The text boxes allow you
to type a brief description of each of the solvents.
Stoptime 0.01 – 99999 minor
As Injector/No Limit
(an infinite run time).
The stoptime sets a time limit for your analysis. After the stoptime, all
gradients are stopped and the pump parameters return to their initial
values. The pump can be used as a stoptime master for the complete
analytical system. The pump also stops the detectors if they have a No
Limit stoptime setting. If no limit is given, a method will have to be
stopped manually.
Posttime 0.01 – 99999 minor
Off ( 0.0 min).
Your instrument remains in a not ready state during the posttime to delay
the start of the next analysis. You can use the Posttime to allow your
column to equilibrate after changes in solvent composition (for example
after gradient elution).
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