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Analytik Jena Biometra TAdvanced Series User Manual

Analytik Jena Biometra TAdvanced Series
140 pages
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Operating Manual
Biometra TAdvanced
High-performance thermal cycler for
DNA amplification by PCR

Table of Contents

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Analytik Jena Biometra TAdvanced Series Specifications

General IconGeneral
Temperature Range4°C to 99°C
Temperature Accuracy±0.1°C
Gradient Range30 °C to 99 °C
ConnectivityUSB, Ethernet
Power Supply100-240 V, 50/60 Hz
DisplayColor touchscreen
Heating Timeapprox. 15 seconds from 50°C to 95°C

Summary

Basic Information

User Manual Notes

Explains conventions, symbols, and signal words used in the manual for hazard indication.

Intended Use

Describes the Biometra TAdvanced as an end-point thermal cycler for nucleic acid amplification.

Safety Instructions

General Safety Notes

General guidelines for safe operation, regulatory compliance, and observed regulations.

Safety Markings on Device

Details warning and instruction signs attached to the device and their meanings.

Technical Condition

Outlines device's technical condition and requirements for safe operation.

Personnel Requirements

Specifies qualifications and training required for operating personnel.

Transport and Installation Safety

Safety instructions for device transport, including packaging and handling precautions.

Operation Safety

Covers safe operation, ventilation, sample handling, and burn risks associated with high temperatures.

Sample Handling Safety

Guidelines for safe handling of samples, auxiliary, and operating materials, including PPE.

Maintenance and Repair Safety

Safety instructions for maintenance, cleaning, and repair procedures, emphasizing authorized personnel.

Emergency Behavior

Instructions on how to react and shut down the device in case of danger or accidents.

Function and Setup

High-Speed Thermoblock and Smart Lid Features

Details thermoblock technology, quick block exchange, and the High Performance Smart Lid (HPSL).

Display and User Interface

Covers the 7" touchscreen, graphical/spreadsheet programming modes, and ease of use.

User-Specific Quick Start Programs

Describes the software's feature for quick access to recently used programs by each user.

Easy Program Creation Methods

Explains options for creating programs: pre-installed templates and the protocol wizard.

Linear Gradient Tool Functionality

Details the tool for programming temperature gradients across the sample block for optimization.

GLP Compliance Features

Mentions user account management and self-test functions for GLP compliance.

Open System Philosophy

Discusses compatibility with various consumables, reagents, and polymerases.

Order Numbers and Configurations

Provides order numbers for different models, block types, and accessories.

Installation and Commissioning

Scope of Delivery and Unpacking Procedures

Lists included items, packaging instructions, and unpacking/inspection steps.

Setting Operating Voltage

Instructions on how to set the device's operating voltage using the selector switch.

General Setup and Network Installation

Covers general setup, ventilation, placement, and network connection procedures.

Control Elements

Front and Rear Panel Views

Identifies and describes the control elements visible on the front and rear of the device.

Quick Block Exchange Mechanism

Explains the procedure for quickly exchanging sample block modules.

High Performance Smart Lid (HPSL) Operation

Details the function of the height-adjustable heated lid and how to close it.

Opening the Heated Lid

Provides instructions on safely opening the heated lid, including reducing contact pressure.

Software Interface: Keyboard and Keypad

Describes the on-screen keyboard and keypad for data entry in the software.

Software Interface: Navigation and Confirmation

Explains cursor keys for navigation and Confirm/Cancel buttons for user input.

Software Commands and Icons Reference

Lists common commands and their corresponding icons used in the Biometra TAdvanced software.

Operation

Power-On Self-Test and Login Screen

Describes the initial self-test upon power-on and the subsequent login screen.

Home Screen Functions Overview

Outlines the main functions accessible from the home screen after user login.

Block Status and Quick Start Functionality

Explains how to check block status (Free, Running, Pause) and use the quick start feature.

Quick Start Program Selection

Details how to use the quick start function for previously used programs.

Incubation Mode Operation

Describes how to use the incubation mode for constant temperature incubation of samples.

Creating, Editing, and Saving Programs

Programming Modes: Graphical vs. Spreadsheet

Explains how to switch between graphical and spreadsheet programming interfaces.

Creating New Programs and Using Templates

Guides on creating new PCR programs or using pre-defined templates.

Assigning Program Names and Setting Lid Temperature

Instructions for naming programs and setting the heated lid temperature.

Preheating the Heated Lid

Details how to enable or disable the preheating function for the heated lid.

Editing Program Steps and Parameters

Overview of parameters that can be entered or modified for each program step.

Editing All Parameters of a Single Step

Step-by-step guide on how to edit all parameters for a selected program step.

Programming Loops and Step Repetitions

Explains how to set up program loops using 'Go To' and 'Cycles' parameters.

Programming Temperature and Time Increments

Details how to add temperature or time increments to program steps within loops.

Adjusting Ramp Rate for Heating/Cooling

Instructions on how to adjust the heating and cooling ramp rates for program steps.

Programming Gradient Steps

Guides on setting up temperature gradients across the sample block for optimization.

Direct Parameter Programming of Steps

Explains how to edit individual parameters directly in the spreadsheet or graphical view.

Inserting and Deleting Program Steps

Procedures for adding new steps or removing existing steps from a program.

Saving Programs to User Directories

Instructions on how to save a program, select a user directory, and storage location.

Starting, Copying, and Deleting Programs

Starting a Program from User Account

Guides on selecting and starting stored programs from a user's account.

Copying Programs (Single and All)

Describes how to copy individual programs or all programs to another location.

Deleting Programs (Single and All)

Instructions for deleting individual programs or all programs from a user's directory.

Running, Pausing, Continuing, and Stopping Programs

Displaying Program Execution Status

Explains how to view program progress in graphical or spreadsheet views.

Pausing and Resuming Program Execution

Details how to pause a running program and then continue it later.

Skipping Steps and Stopping Programs

Covers how to skip a program step or completely stop the program execution.

Protocol Wizard

Selecting Polymerase and Creating Programs

Guides on using the wizard to create PCR programs based on polymerase templates.

Editing Polymerase Templates

Instructions on how to enter new polymerases or edit existing preset program templates.

Deleting Polymerase Templates

Explains how to remove polymerase templates from the software.

Protocol Wizard Parameter Configuration

Outlines parameters for automatic PCR program generation, including product length and cycles.

Primer Annealing Temperature Calculator

Describes the tool to calculate theoretical primer annealing temperatures based on sequences.

Tools

System Configuration Settings

Covers basic device settings like date, time, logout, beeper, network, and calibration.

User Management Operations

Explains how to manage user accounts, set rights, and create new users.

Documentation and Log Files

Details access to run logs, power-on logs, self-test logs, and error logs.

Extended Self-Test Functionality

Describes the process and components checked during the extended self-test.

Service Tools and Device Information

Explains creating service files, backup functions, and accessing device information.

Contacting Biometra Service

Provides contact details for the Biometra service department.

Adaptation of Programs

Quick Reference Guide

Login and Home Screen Operations

Provides a quick overview of login procedures and home screen functions.

Program Editing and Management

Summarizes steps for editing, saving, copying, and managing PCR programs.

Program Execution Control

Quick guide on starting, pausing, continuing, skipping, and stopping programs.

Fault Removal

Password Issues and Performance Problems

Addresses forgotten administrator passwords and issues like slow heating/cooling.

Auto Restart and Program Adaptation

Explains auto-restart behavior and adapting programs from other cyclers.

Lid Wheel Issues and 384-Well Block Usage

Covers troubleshooting lid wheel blockages and recommendations for 384-well blocks.

Maintenance and Care

Cleaning the Device Housing

Instructions on how to clean the exterior of the Biometra TAdvanced safely.

Disinfecting the Device and Sample Block

Details the procedures for disinfecting the device and sample block, especially for infectious materials.

Firmware Update Procedures

Information on how to obtain and perform firmware updates for the device.

Transport

Device Return Information and Decontamination

Guidelines for returning the device, including decontamination and documentation requirements.

Packing Instructions for Shipment

Describes the proper packaging procedure to prevent transport damage.

Disposal

Specifications

Model-Specific Performance Data

Provides detailed specifications for different Biometra TAdvanced models.

General Specifications for All Models

Lists general technical specifications applicable to all models of the device.

Declaration of Conformity

Index

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