Rev 9/20/18 pg. 93
Appendix C – De-fatting Procedure
If de-fatting is desired, please follow the procedure below using the ANKOM XT4 filter bag.
The XT4 Filter Bag is used in the ANKOM XT Fat Extractors. The bag has a
porosity of 1-4 microns. As a result, the likelihood of sample loss is eliminated.
To de-fat your sample prior to running the IDF/SDF or TDF analyses, follow the steps below.
Number all the XT4 Filter Bags to be used for testing.
Record the weight of each XT4 Filter Bag.
It is important to note that the weight of the XT4 Filter Bag must be included in
the analysis calculations in the following ways:
• For an IDF/SDF analysis, add the weight of the XT4 bag to the weight of
the IDF bag.
• For a TDF analysis, add the weight of the XT4 bag to the weight of the
SDF bag.
Record the weight of each sample to be de-fatted in the appropriate
XT4 Filter Bags.
Heat seal the Filter Bags.
With the heat sealer set at 5-6, seal the Filter Bag within 4mm of its open end. Be sure
to hold the sealer arm down for 2-3 seconds after the red sealer light turns off to
properly cool the seal.