Aspirating and Dispensing
Sample Solution
Using a plastic transfer pipette, transfer an aliquot of Sample
Solution into an aliquot container. Both items are located in
the Reagent Kit. Transfer only enough solution for half-hour
increments. The volume gradations on the transfer pipette
and the 1 mL mark on the aliquot container can be used as
a guide for the volume of solution transferred.
• Aspirate the Sample Solution from the aliquot container
with the pipette under test.
• Lift the vial holder cover and dispense the Sample Solution
into the Blank vial using standard pipetting technique.
Important Note:
The Sample Solution should be dispensed against the inside wall of the vial with the pipette tip placed above the
meniscus but below the shoulder of the Blank vial. The vortex mixing action will wash the solution from the inside
wall of the vial.
Pipette
Calibration
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