The Ultrospec 7500 Spectrophotometer is a UV/Visible split-beam instrument designed for accurate and reliable measurement of light transmission through samples. It is widely used in modern laboratories across various fields, including life science, clinical, healthcare, industrial research, quality control, manufacturing, agriculture, animal husbandry, fishery, geological exploration, food safety, and environmental monitoring. The device is known for its quick and accurate scanning operating system, making it suitable for identifying and quantifying organic and inorganic compounds.
Function Description
The Ultrospec 7500 measures the transmission of light through a sample, with absorbance being directly proportional to the sample concentration and pathlength. It is primarily used for:
- Single Wavelength Measurements: Performing simple absorbance (A) and % transmission (%T) measurements, and determining sample concentration using a known factor or a standard of known concentration.
- Wavescan: Performing absorbance (A) or % transmission (%T) measurements across a range of wavelengths to create an absorbance or transmission spectrum. This application also includes feature detection for identifying peaks and valleys in the spectrum.
- Kinetics: Performing a series of absorbance (A) measurements over a defined timeframe to create a time-course trace, allowing for the study of reaction rates.
- Standard Curve: Creating calibration curves from standard samples of known concentration. The curve fit equation is then applied to subsequent test samples to determine their concentration.
- Substrate: Similar to Standard Curve, this application creates a calibration curve from kinetic measurements of samples of known concentrations, with the curve fit equation applied to subsequent test samples.
- Equation Editor: Creating more complex custom methods incorporating bespoke calculations based on various measurement parameters.
- Protein UV: Performing protein quantification measurements at 280 nm and assessing sample quality using A260/A280 and A260/A230 absorbance ratios.
- Colorimetric Protein: Performing Biuret, BCA, Bradford, Lowry, and Pierce protein quantification assays.
- Protein Dye: Assessing the fluorescent labeling efficiency of protein probes based on absorbance, prior to their use in microarrays.
- DNA: Performing DNA quantification measurements at 260 nm and assessing sample quality using A260/A280 and A260/A230 absorbance ratios.
- RNA: Performing RNA quantification measurements at 260 nm and assessing sample quality using A260/A280 and A260/A230 absorbance ratios.
- Oligo: Performing Oligonucleotide quantification measurements at 260 nm and assessing sample quality using A260/A280 and A260/A230 absorbance ratios.
- Fluorescent Dye: Assessing the fluorescent labeling efficiency of nucleic acid probes based on absorbance, prior to their use in microarrays.
- OD 600: Performing simple optical density measurements of microbial growth cultures.
- Tm Calculation: Determining the theoretical melting point (Tm) of a PCR primer at a measured concentration from its nucleotide base sequence, using the nearest-neighbor model.
Important Technical Specifications
- Wavelength Range: 190 to 1100 nm
- Monochromator: 1200 lines/mm Aberration corrected concave grating
- Wavelength Calibration: Automatic upon switch on
- Beam Height: 15 mm
- Spectral Bandwidth: <2 nm
- Wavelength Accuracy: ±1 nm
- Wavelength Reproducibility: ±0.5 nm
- Light Sources: Xenon flash lamp
- Detector: Two silicon photodiodes
- Photometric Range: -3.000 to 3.000 A, 0.1 to 100000 %T
- Photometric Accuracy: ±0.5 % or ±0.003 A whichever is greater at 546 nm
- Photometric Reproducibility: ±0.5 % to 3.000 A at 546 nm
- Stray Light: <0.05 %T at 220 nm using NaI or at 340 nm using NaNO2; <0.10 %T at 380 using NaNO2
- Stability: ±0.001 A/h at 340 nm for 0 A
- Noise: ±0.002 peak to peak ± 0.0005 RMS at 340 nm for 0 A
- Digital Output: USB Flash Drive, PC via PVC software
- Data Export: USB Flash Drive: .tsv, native PVC format; PC via PVC: .csv, .emf, .xlsx, .xls, .rtf, .tsv, native PVC format
- Method Storage: 156 with PIN number protection
- Graphical Display: Yes, zoom and track function
- Sample ID: Yes
- Languages: English, German, French, Spanish, Italian, Japanese, Chinese
- Dimensions: 510 × 350 × 160 mm
- Weight: 13.00 kg
- Power Input: 19 VDC at max 90 VA from a supplied 100 to 240 V~, 50/60 Hz Mains Power Adapter
- Optical Height (z value): 15 mm
- Cell Changer: Standard 8-position cell changer for 10 mm pathlength quartz, glass, or plastic cuvettes. Alternative accessories are available.
Usage Features
The Ultrospec 7500 features a user-friendly interface with a colour touchscreen display (800 × 480-pixel resolution) for navigation.
- Onscreen Keyboards and Number Pad: Facilitates data entry and parameter adjustments.
- Intuitive Icons: Frequently used icons for navigation, measurement, options, and settings simplify operation.
- First-time Start-Up: Guides users through self-calibration, regional settings, and date/time setup upon initial power-on.
- Home Screen: Provides icon-based access to all applications, settings, user access controls, and instrument information.
- User Access Control: Allows for managing user logins, passwords, and permission groups (Administrator, Supervisor, Limited) for enhanced security and data integrity.
- GLP Settings and Application: Defines GLP routine preferences, views the latest GLP report, prints, saves, and reruns GLP reports, and indicates GLP errors if test parameters are out of limits.
- Instrument Status: Provides access to instrument information, settings, lamp settings, and reset options.
- Data Output Options: Allows for auto-printing and auto-saving of data to an internal printer, PC via USB, or USB flash drive in various formats (.tsv, native PVC, .csv, .emf, .xlsx, .xls, .rtf).
- Sample Management: Features a Sample Manager screen to save, load, lock, and delete result data.
- Method Management: Allows saving custom methods to directories (Methods, Favourites, USB Methods), with options to open, delete, lock, unlock, and rename method folders. Methods can also be backed up to or restored from a USB memory stick.
- Screenshot Functionality: Enables taking screenshots of any screen and saving them as .BMP files to a USB memory stick.
- Cell Changer: The standard 8-position cell changer simplifies batch measurements. The instrument can be configured to use a single cell holder or a cell changer, with options for prompting per sample and setting the number of samples.
Maintenance Features
- Self-Diagnostic Checks: Performs a series of self-diagnostic checks upon power-on.
- Lamp Settings: Provides access to view the age of the Xenon lamp.
- Instrument Reset: Allows for deleting all user data, including samples, logins, methods, and method folder names.
- Baseline Management: Enables creating new temporary baselines, saving them as permanent baselines, and restoring temporary baselines from stored permanent ones.
- Troubleshooting Guide: Includes a comprehensive troubleshooting section for common issues such as negative absorbance readings, unexpected results, high/low absorbance, poor reproducibility, and instrument start-up failures.
- Technical Support: Provides contact information for technical support in the US, UK, and China, with online returns forms and email support.
- External Printer Installation Guide: Detailed instructions for installing the optional Seiko DPU S245 thermal printer, including connecting cables and performing test prints.
- Decontamination Procedures: Recommendations for decontaminating the unit, including wiping exterior casework, washing cuvettes and holders, and proper disposal of hazardous materials.
- Safety Notes: Emphasizes potential hazards, such as Xenon lamp energy and high voltages, and advises that repairs and maintenance should only be carried out by trained individuals. It also highlights the importance of safe working environments and proper handling of chemicals and biohazardous samples.
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