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correct type. Some cells absorb in the UV and are not suitable for UV sample measurement.
Cells used for samples should be free from dust, residue or scratches.
Before preparing samples and sample reference blanks, you must be familiar with hazards
arising from handling the sample materials and where necessary observe local regulatory
practice, personnel protection equipment and measures designed to ensure your safety.
Prepare the sample blanks (references). A reference is typically the solution that the sample
is dissolved in. For Split beam models a reference is required. For Dual beam units the
reference may be placed in the reference path or a separate sample reference may be made
by first placing the reference in the sample path and performing a reference measurement.
Prepare the sample solutions. The sample solution would normally comprise the sample
under test dissolved in the reference solution
When placing the cells in the equipment ensure the cell is orientated so that the light energy
will pass through the cell
For further information on running applications and methods refer to the user manual
Post Run procedures
Empty cuvette cells of sample and rinse with deionised water
Clean cuvettes periodically with commercially available cleaning solution or dilute detergent
solution followed by a thorough rinse in deionised water.
Note that some samples and solvents may be classified as hazardous or bio hazardous
waste. The disposal of such substances must be carried out in accordance with local
regulatory practice
Performance Validation
Good laboratory practice recommends that the unit is periodically checked for optical performance.
Switch on validation checks. When the unit is powered up it performs wavelength accuracy
and lamp energy checks. Once complete the unit will beep and show a green on light.
Periodically wavelength, stray light and absorbance should be checked to ensure the unit is
performing to specification. Deterioration in performance may indicate that the instrument
needs servicing or that a poor baseline has been saved. Performance validation can b carried
using traceable reference materials.
User Maintenance
Other than the Tungsten and Deuterium lamps in certain instruments there are no user
serviceable parts in this equipment.
To prevent contamination
o Cell holders and accessories should be removed and cleaned with commercially available
cleaning solution or dilute detergent followed by a through rinse in deionised water.
Allow to dry thoroughly before use
o Casework and the sample compartment may be wiped down with commercially
available disinfectant wipes
The lamps used in the unit age over time and less energy will be available to pass through
the sample. Where energy has fallen to around 50% of the installation energy you are
If the equipment is operated in a manner not specified, then the protection provided by
the equipment may be impaired and the instrument warranty withdrawn.
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