Biocomp GRADIENT MASTER 108 - Programming Instructions; Levelling the Plate; Recalling a Gradient from Memory; Preparing and Capping Gradient Tubes

SECTION 6. PROGRAMMING INSTRUCTIONS
A. LEVELLING THE PLATE: A CRITICAL FIRST STEP
There are two Levelling functions:
1. When you power up the device (see above)
2. Levelling the plate during use. Press the EXIT key until the display shows the Main Menu;
GRADIENT MASTER108v5.22
GRAD SYST
Press SYST and the press LEVL and repeat the procedure as above. When done, press EXIT to return
to the main menu.
B. RECALLING A GRADIENT FROM MEMORY AND RUNNING IT
NOTE: The Gradient Master is preprogrammed to run your gradients. You do not need to download
any software. The SD card provided is only for backup.
1. When the plate is level, press the DONE key and the display should read:
GRADIENT MASTER108v5.22
GRAD SYST
Press GRAD:
GRADIENT MENU
LAST RCNT LIST NEW EXIT
If you are using a new gradient for the first time, you have two options. You can find it in the LIST, or
you can enter it into the memory (NEW). To locate it in the LIST, press the LIST key:
Select Rotor, Use Arrow
<--> SW41 EXIT
The rotors you purchased tube holders for should appear. If more than three rotors are loaded, press
the arrow key to shift the display until your rotor appears.
Press the key under the rotor you want to use:
Short Sucr 02-28% wv
DOWN UP USE DEL EXIT
The top line summarizes the gradient: Short is the short cap, used for rate zonal runs, Sucr is the
solute, 02-28% wv are the top and bottom percentages of the solute with the measurement units.
The EXIT key can be used at any time to stop the current run or move one level up toward the Main
Menu with each stroke.
The LIST of gradients is stored in memory in a fixed order: First all the Short cap gradients will be
displayed, followed by the all the Long cap gradients. For the short cap, all the sucrose gradients are
listed first, followed by glycerol and then the exotic gradients such as Percoll, Nycodenz, NaCl etc.
Within each solute, the gradients are organized first by lower percent, then high percent then by
measurement (see the gradient list at the back of the manual). Thus, all the 5% gradients are listed
before the 12%, which are listed before the 15%, etc. Press and hold the DOWN and UP keys to scroll
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