1. CALIBRATION
Each pipette has been checked & calibrated at factory with procedure conforming to EN- ISO
8655-6 standards.
Depending upon use, we recommend checking of calibration every six months. However this can be
adjusted to individual requirements.
1.1 Procedure to check calibration
The pipette is checked at maximum volume, at 50 % of maximum volume and at minimum or 10%
of maximum volume, whichever is higher.
• A new tip is first pre-wetted 3-5 times and a series of ten pipetting is done at each volume.
• Use of forward pipetting technique is recommended.
• Calculate the inaccuracy and imprecision for all three volumes as per EN ISO 8655-6/DIN
12650 standards based on the following calculation
1.2 Conversion of weight readings to volume
• Mean Value
• Mean Value
• Xi: Balance Reading
• n: Number of Reading
• Z : Conversion factor [example Z=1.0040 µl/mg at 25°c and 1013 hPa]
1.3 Calculation for in-accuracy (Systematic Error)
•
• V: Mean Value
• Volume at which readings are taken
1.4 Calculation for Imprecision (Random Error)
•
•
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