• S: Standard Deviation
•
Mean Value
• n: Number of readings
1.5 Devise requirements and test conditions
An analytical balance must be used. The balance selection depends upon selected model of the
pipette and sensitivity of balance reading.
Test liquid: Water, distilled or deionized, grade 3 water conforming ISO3696.
Calibration should be carried out in a draft- free room at a constant (+/- 0. 5 °C) temperature of
water, pipette and air between 15°C to 30°C. The relative humidity must be above 50% especially
with volumes under 50 µl, the air humidly should be as high as possible to reduce the effect of
evaporation loss.
Special accessories for analytical balance, such as the evaporation trap are recommended for the
calibration of volumes under 50 µl.
1.6 Recalibration
1. Place the service tool intro grooves at the base of the push button as shown below. Turn it
clockwise to increase & anticlockwise to decrease the volume.
2. Repeat the ‘Checking calibration’ procedure.
Fig 1: Recalibration procedure
2. Storage
When not in use, it is recommended that your pipettor is stored in a vertical position.
Leaving the pipette on it’s side can cause liquids to leak into the body of the pipette and cause
corrosion.
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