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USER MANUAL RETEVET™ PORTABLE ERG
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In the protocol Cone function testing, only light-adapted tests are performed. At least 10 minutes exposure to the ambient
light close to 30 cd/m² (outside or examination room) should be done before testing. This protocol is recommended for quick,
cone-based general assessment of the retinal function, for example, for pre-cataract testing. We also recommend this
procedure for the inexperienced users.
Cone function testing
Description Eye Flash Luminance Energy
(0.33, 0.33 White)
Background Luminance
(0.33, 0.33 White)
# Flashes
Light adaptation timer Right Off 30 cd/m²
Light adapted 3.0 Right 3 cd·s/m² @ 2 Hz 30 cd/m² 20
Light adapted 3.0 fl icker Right 3 cd·s/m² @ 28.3 Hz 30 cd/m² 141 – 424
Light adaptation timer Left Off 30 cd/m²
Light adapted 3.0 Left 3 cd·s/m² @ 2 Hz 30 cd/m² 20
Light adapted 3.0 fl icker Left 3 cd·s/m² @ 28.3 Hz 30 cd/m² 141 – 424
In the protocol ECVO long protocol for PRA diagnosis, dim fl ash ERGs are performed during dark adaption in order to assess
the rate of rod recovery in the dark. Measurements occur every 4 minutes starting at time 0. The choice of which eye to test
eye is offered at the beginning of the test, as only one eye is tested. Light adaptation of 10 minutes occurs after the right and
left eyes dark-adapted tests are completed. Waiting or examination room ambient light close to 30 cd/m² is likely suffi cient
or one can use the built-in light-adaptation 10-min period. This protocol is recommended for anesthetized or sedated animals
when the progression of dark-adaptation needs to be evaluated, for example in Progressive Retinal Atrophy (PRA) (Miyadera,
Acland, and Aguirre 2012). PRA is a bilateral disease so that evaluating one eye is suffi cient (Ekesten et al. 2013).
ECVO long protocol for PRA diagnosis
Description Eye Flash Luminance Energy
(0.33, 0.33 White)
Background Luminance
(0.33, 0.33 White)
# Flashes
Dark-adapted 0.01 Operator’s choice 0.01 cd·s/m² @ 0.2 Hz Off 4
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 4
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 4
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 4
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 4
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 4
Dark-adapted 3.0 3 cd·s/m² @ 1 / 15 Hz Off 4
Dark-adapted 10.0 10 cd·s/m² @ 0.05 Hz Off 1
Light adaptation timer Off 30 cd/m²
Light adapted 3.0 3 cd·s/m² @ 2 Hz 30 cd/m² 20
Light adapted 3.0 fl icker 3 cd·s/m² @ 28.3 Hz 30 cd/m² 141
– 424
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