6-16 COBE 2991 Cell Processor • Essentials Guide
Processing and Common Operations
Loading Cell Product with no Predilution (Hematocrit of 35% or Less)
Use the following procedure to load the starting cell product without predilution.
1 Press the BLOOD IN button. Valve V1 opens, allowing the starting cell
product to flow by gravity into the cell processing bag.
After a short time, the starting cell product stops flowing. Depending on the
initial volume, some starting cell product may be left in the cell product bag.
2 Set the CENTRIFUGE SPEED control to 3000 rpm, then press the AIR
OUT button. Valve V1 remains open and the centrifuge spins, pushing air and
starting cell product back up the red-striped tubing through valve V1 to the
cell product bag.
3 When air and starting cell product reach the cell product bag, press the
BLOOD IN button again. Additional starting cell product enters the cell
processing bag.
4 When no more fluid will flow into the cell processing bag or the fluid level
(
Figure 6-15) is just above the rotating seal, press the STOP RESET button.
The capacity of the cell processing bag is approximately 630 mL. If the volume
of the starting cell product is more than 630 mL, not all of the starting cell
product will flow into the cell processing bag during the Blood-In/Air-Out
sequence. Additional starting cell product may be added during subsequent
Agitate/Wash-In operations by programming valve V1 to open instead of
valves V2 or V3. Add additional wash cycles as needed.
Caution: If
using an external
pump to deliver
fluid to the cell processing
bag, monitor the volume
added to the cell
processing bag. This is
necessary to prevent
exceeding bag capacity
and potentially
compromising the cell
processing set.
Caution: Do
not press STOP
RESET during an
Air-Out operation. This
could lead to excess
pressure in the cell
processing bag, which may
damage the rotating seal.
Caution:
Verify that fluid is
visible above the
rotating seal when the
centrifuge is spinning to
ensure adequate seal
lubrication. Inadequate
rotating seal lubrication
can lead to failure of the
rotating seal and potential
contamination or loss of
cell product.
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