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gloves. This is accomplished by discharging approximately 30% of the total filtered air each cycle
through the exhaust HEPA filter in the top of the unit.
An equal amount of make-up air is pulled into the chamber through the bottom of the window
under negative pressure from the blower and positive pressure from the air curtain. The viewing
panel has a unique, recessed, contoured shape, which creates an aerodynamic flow pattern. It is this
combination that produces the protective barrier for the operator by containing hazardous materials
within the chamber.
The BENCHTOP BIOFLOW CHAMBER exceeds the requirements for a class 100 chamber
described in Federal Standard No. 209. This standard outlines air cleanliness classes and certain
other environmental air conditions required for achieving and maintaining the levels of
environmental cleanliness. Class 100, according to Federal Standards, is the class where particles 0.5
micron or larger must not exceed a total count of 100 particles per cubic foot. Biomedical
engineering and technology have introduced safeguards into the design of laboratory equipment, but
these safeguards do not prevent human error. Danger to personnel from carelessness or improperly
used equipment cannot be overemphasized. There is no substitute for safe, proper technique.