illumina MiSeq System Guide

MiSeq Workflow
Prepare the prefilled reagent cartridge for use.
Denature and dilute libraries (does not apply to all library types). See Preparing
Libraries for Sequencing on the MiSeq (document # 15039740) .
Load the library mix onto the reagent cartridge in the designated reservoir.
From the software interface, select Sequence to start the run setup steps.
From the Sequence Mode Selection screen, select a run setup option: Local
Run Manager, Sample Sheet, or Manual.
[Optional] From Local Run Manager selection screen select a run.
[Optional] Connect to BaseSpace or BaseSpace Onsite.
[Optional] From the Sample Sheet selection screen, browse to select a sample
sheet for the run.
[Optional] Connect to BaseSpace or BaseSpace Onsite.
[Optional] Form the Manual mode screen, manually enter the run parameters.
No secondary analysis is available in this mode.
Wash and thoroughly dry the flow cell.
Load the flow cell.
Load the PR2 bottle and make sure that the waste bottle is empty.
Load the reagent cartridge.
Review run parameters and pre-run check results.
Select Start Run.
Monitor your run from the MCS interface or from another computer using Local
Run Manager or Sequencing Analysis Viewer (SAV).
Document # 15027617 v04 Material # 20024228
For Research Use Only. Not for use in diagnostic procedures.
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MiSeq System Guide

illumina MiSeq Specifications

General

Brand	illumina
Model	MiSeq
Category	Recording Equipment
Language	English