Labnet BioPette PLUS - Page 18

16 – LABNET BIOPETTE PLUS PIPETTORS
Checking the Pipetting Accuracy Parameters
• Set the preset volume depending on the pipettor
volume according to the following table.
Nominal
volume
(µL)
Preset
volume
(μL)
Permissible
values (μL)
Volume Change ΔV (μL) for a
Turn of a Calibration Key by
1 Turn 1 Increment
Single-channel Pipettors
2 0.2 0.176 - 0.224 0.06 0.0025
10 1 0.975 - 1.025 0.33 0.0137
20 2 1.94 - 2.06 0.63 0.0262
100 10 9.84 - 10.16 2.50 0.104
200 20 19.76 - 20.24 6.30 0.262
1,000 100 98.4 - 101.6 25.00 1.04
5,000 500 494 - 506 125.00 5.2
10,000 1,000 975 - 1,025 250.00 10.4
8-channel and 12-channel Pipettors
10 1 0.92 - 1.08 0.33 0.0137
50 5 4.8 - 5.2 1.67 0.070
200 20 19.4 - 20.6 6.30 0.262
300 50 49.2 - 50.8 10.00 0.42
• Perform 10 aspirations, and calculate the average value
in [mg].
• Calculate the volume in [μL] by multiplying the value
in[mg] by the conversion factor Z [μL/mg].
If the average aspirated volume exceeds the permissible
values of the range, the pipettor should be recalibrated.