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Labnet ENDURO VE10 - User Manual

Labnet ENDURO VE10
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Copyright 2017 Labnet International, Version # 2
ENDURO™ VE10 Vertical Gel Electrophoresis Systems
User Manual
ENDURO VE10 System
E2010-PA
ENDURO VE10 Electroblotting System
E2010-PBA
Lit #M00608
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Summary

Safety Precautions

Care and Maintenance

Cleaning Products and Accessories

Details cleaning agents and methods for the units.

Packing Lists

ENDURO VE10 Vertical Gel System Packing

Lists accessories included with the ENDURO VE10 Vertical Gel System.

ENDURO VE10 Electroblotting System Packing

Lists accessories included with the ENDURO VE10 Electroblotting System.

Usage Guidance and Restrictions

System Care and Maintenance

Questions and Service

RNase Decontamination Protocol

Vertical Gel Casting

Assembling Glass Plates

Steps for cleaning and assembling glass plates for gel casting.

Securing Gel Insert in Casting Base

Procedures for placing and securing the gel insert in the casting base.

Using the Casting Stand Cams

Instructions on operating the casting stand cams for proper sealing.

Gel Preparation

Preparing Resolving Gel Solution

Instructions for preparing the resolving gel solution using stock solutions.

Gel Selection

Guidance on choosing gel percentage based on protein size separation.

Gel Pouring

Pouring Discontinuous Gels

Steps for pouring discontinuous gels into the assembled plates.

Pouring Continuous Gels

Instructions for preparing and pouring continuous gels.

Preparation of denatured protein samples for loading

Loading the Samples

Procedures for loading prepared protein samples into the gel wells.

Gel Running

Setting up for Gel Running

Instructions for fitting the lid, connecting power, and filling buffer.

Running the Gel

Parameters and steps for running the gel electrophoresis.

Recommended Voltage and Current Settings

Protein Blotting

Setting up the Cassette Sandwich

Steps to assemble the blot sandwich for transfer.

Blot Running Conditions

Buffer Volume Requirements

Table detailing buffer volumes needed for different cassette configurations.

Recommended Voltage and Current Settings

Appendix

Stock Solutions for SDS PAGE gels

Recipes for preparing various stock solutions for SDS PAGE.

Membrane Selection

Information on different membrane types for blotting applications.

Buffer Preparation - Proteins

Towbin Buffers

Recipes for native and denatured gel Towbin buffer solutions.

Warranty Statement

Accessories

Labnet Accessories

List of available accessories with catalog numbers and descriptions.

Plates and Spacers

Details of available glass plates and spacers.

Combs

Specifications and types of available gel combs.

Labnet ENDURO VE10 Specifications

General IconGeneral
Max Voltage300 V
Dimensions28 x 20 x 12 cm
Temperature ControlNo
Gel Size10 x 10 cm
MaterialsPlastic and metal
Frequency50/60 Hz
CapacityNot Applicable
MaterialPlastic and metal

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