16
Protein Blotting using the ENDURO VE10 Electroblotting Insert
Setting up the cassette sandwich: (Common buffer solutions are listed in the
Appendix.)
1. Each blot sandwich should be set up as follows:-
a. Cassette clamp -ve (black) side placed in a tray or other suitable surface.
b. Pre-soaked fiber pad.
c. Two pieces of .45µm filter paper, pre-soaked in buffer.
d. Gel - cut the left hand corner for indexing,
e. Transfer membrane. Follow manufacturer’s directions for presoaking.
Smooth out any air bubbles that may be trapped under the membrane.
f. Two pieces of filter paper, pre-soaked in buffer.
g. Pre-soaked fiber pad.
h. Cassette clamp +ve (red) side slotted into the groove in the bottom of the
black cassette.
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