Labnet ENDURO VE10 - Gel Preparation; Preparing Resolving Gel Solution

9
Vertical Gel Casting:
A) Screw Version B) Sliding clamp version
Gel Preparation:-
1. Stock solutions for SDS PAGE gels should be prepared prior to use and chilled.
For native gel formulas and running conditions, please consult the appendix. The
protocol below is given for use of the standard stock solutions.
2. Table 1 below shows the total volume of gel solution required. In subsequent
tables, amounts of gel and solutions are given for two 1mm thick gels. If gels of
other thickness are to be run adjustments will need to be made.
1) Put together bonded
spacer plain glass plate
with notched plate
3A) Fully tighten
screws ensuring
not to wobble unit.
2)
Insert
inside
pressure
bar with notched plate
innermost touching the
gasket and module on a
flat surface away from the
casting base
3B) Fully slide
clamps tight
ensuring not to
wobble unit.
4) Insert into casting
base. Push the cams
into the holes in the
insert. Turn cams
about 90
0
or until
tight. Do not over
tighten.
5) Pour resolving gel
and allow to set.
Then stacking gel
solution and insert
comb.
6) Once set, transfer
to tank and fill inner
and outer chambers
with buffer.