Labnet ENDURO VE10 - Buffer Preparation - Proteins; Towbin Buffers

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Buffer Preparation - Proteins
Towbin Buffers
Native Gels
Towbin Buffer pH 8.3
25 mM TRIS, 192 mM glycine, 20% Methanol,
3.0 gm TRIS
14.4 gm glycine
200 ml Methanol
add ddi H
2
O to 1 liter
Denatured Gels
Towbin Buffer pH 8.3, no Methanol
25 mM TRIS, 192 mM glycine
3.0 gm TRIS
14.4 gm glycine
add ddi H
2
O to 1 liter