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5. When the blot time is completed, turn the power supply off and remove the lid of
the unit.
6. Remove the cassettes from the main tank.
7. Lift the hinge of each cassette and gently pry apart the blot sandwich and remove
the membrane from the gel.
8. The membrane can now be further processed. Remember to save the filter paper
behind the gel to check for blow through of smaller proteins.
Table 9. Recommended voltage and current settings.
One Hours 100V, 400mA
Three Hours 50V, 200mA
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