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Leica CM 1850 User Manual

Leica CM 1850
54 pages
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Instruction Manual
Leica CM 1850 V2.0 English – 03/2001
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica CM 1850
Cryostat

Table of Contents

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Leica CM 1850 Specifications

General IconGeneral
BrandLeica
ModelCM 1850
CategoryMedical Equipment
LanguageEnglish

Summary

1. Important Information

1.1 Symbols and Warnings

Explains warning symbols and notes used throughout the manual.

1.2 Designated Use

Defines the intended applications and scope for the Leica CM 1850 cryostat.

1.3 Personnel Qualification

Specifies the required training and qualifications for operating the instrument.

2. Safety

2.1 Safety Features

Details safety mechanisms like handwheel locking and knife guards.

2.2 General Handling and Design Safety

Covers safe design principles and general handling precautions.

2.3 Operating Conditions

Outlines essential environmental and connection requirements for safe operation.

2.4 Operating the Instrument Safely

Provides critical instructions for safe instrument usage and handling.

2.5 Cleaning and Disinfection Safety

Covers safe procedures for cleaning and disinfecting the cryostat.

2.6 Microtome Removal Safety

Details safety precautions for removing the microtome from the cryochamber.

2.7 Maintenance Safety

Highlights safety aspects related to fuse and lamp replacement.

3. Technical Data

3.1 Refrigeration System Specifications

Details temperature ranges, defrosting methods, capacity, and refrigerant.

3.2 Microtome and Lamp Specifications

Lists specifications for the microtome, specimen feed, and fluorescent lamp.

3.3 Cryocabinet Dimensions and Weight

Provides physical dimensions and overall weight of the cryocabinet.

3.4 Fuse Specifications

Lists the required fuse types and ratings for the instrument.

4. Unpacking and Installation

4.1 Site Requirements

Specifies environmental and space conditions for instrument installation.

4.2 Transporting the Instrument

Instructions on how to safely move the cryostat to its installation location.

4.3 Standard Delivery Contents

Lists all items included in the standard delivery package for the CM 1850.

4.4 Handwheel Assembly

Step-by-step guide for attaching the handwheel to the instrument.

5. Setup

5.1 Mains Power Connection

Details requirements and precautions for connecting the instrument to power.

5.2 Prior to Operation Checks

Lists essential steps to perform before initial instrument operation.

5.3 Leica CM 1850 Overview

Diagram and identification of key components of the CM 1850 cryostat.

5.4 Mains Switch and Fuse

Explains the function and operation of the mains switch and fuse.

5.5 Turning the Instrument On

Procedures and important notes for powering up the cryostat.

6. Control Panel Operation

6.1 Control Panel 1 Functions

Describes the buttons and display of Control Panel 1.

6.2 Programming Desired Values

Covers setting time, defrost, temperature, Peltier element, and manual defrost.

6.3 Display Lock Function

Explains how to lock and unlock the control panel to prevent modifications.

6.4 Control Panel 2 - Electric Coarse Feed

Details the operation of the electric coarse feed controls.

7. Daily Use of the Instrument

7.1 Specimen Freezing

Guides on preparing and freezing specimens using the cryostat.

7.2 Specimen Disc Handling

Covers inserting specimen discs and orienting specimens for sectioning.

7.3 Sectioning Procedure

Details trimming specimens and setting section thickness for optimal results.

7.4 Temperature Selection Chart

Provides recommended cryochamber temperatures for various tissue types.

7.5 Defrosting Procedures

Explains automatic and manual defrosting cycles for the cryochamber.

7.6 Terminating Work

Outlines steps for safely shutting down the instrument after daily use.

8. Troubleshooting

8.1 Error Messages and Display Codes

Lists potential error codes and their descriptions displayed on the instrument.

8.2 Temperature Control Button

Explains the function and troubleshooting related to the temperature control button.

8.3 Common Problems and Remedies

Provides solutions for frequent issues like frost, sectioning defects, and ice formation.

9. Cleaning, Disinfection, and Maintenance

9.1 Daily Cleaning Procedures

Outlines daily tasks for maintaining instrument cleanliness.

9.2 Spray Disinfection

Details the process for disinfecting the cryostat using Leica Cryofect.

9.3 Maintenance and Component Replacement

Covers general maintenance, component removal, reinstallation, fuses, and lamps.

10. Ordering Information and Accessories

10.1 Ordering Information

Lists part numbers for knife holders, specimen discs, accessories, and consumables.

10.2 Optional Accessories

Describes optional items like heat extractors and thermal blocks.

11. Appendix

Product Changes and Warranty Information

Details Leica's policy on product modifications and warranty terms.

Disposal and Technical Service

Provides guidelines for instrument disposal and contacting technical support.

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