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Leica DM L User Manual

Leica DM L
75 pages
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Leica DM L
Instructions
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Leica DM L Specifications

General IconGeneral
Microscope TypeCompound
IlluminationHalogen or LED
FocusingCoaxial coarse and fine focusing
Eyepiece10x
StageMechanical stage with X/Y movement
CondenserAbbe condenser

Summary

Important notes on this manual

Installation and safety

Safety

Crucial safety advice for operating the microscope and its accessories safely.

Installation site

Guidelines for selecting an appropriate workplace, considering environmental factors.

Power supply

Built-in power supply

Details on different built-in power supply types and their voltage settings.

Fuses

Information regarding the safety fuses used in the microscope.

Assembly of condensers

Condensers CL/PH and CLP/PH and UCL/UCLP

Details on specific condenser types required for polarization techniques.

Assembly of the condenser disc

Fixing the condenser

Steps for correctly inserting and fixing the condenser into its mount.

Assembly of stage, object guide, tube system

Stages

Instructions for assembling mechanical stages on DMLB and DMLM microscopes.

Tube and intermediate systems

Guidance on adapting tubes and intermediate systems to the microscope stand.

Eyepieces

Eyepieces for observation

Details on various HC PLAN and widefield eyepieces for visual observation.

Objectives

Lamps, assembly

Safety warnings for lamps

Highlights hazards like fire and glare when handling lamps.

Lamphousing 106 z* Hg and Xe lamps

Safety Precautions for Hg and Xe Lamps

Crucial safety measures for handling Hg and Xe lamps, including explosion risks.

Burner Insertion and Collector Exchange

Burner Insertion Instructions and Safety Checks

Performance parameters of objectives

Transmitted light, basic setting

Focusing

Diaphragm settings

Aperture diaphragm for fluorescence

Setting the aperture diaphragm for fluorescence and other techniques.

Operation of transmitted light

Operation of objectives

Immersion objectives

Guidelines for using immersion objectives with oil or water.

Operation of phase contrast

Immersion condensers for phase contrast

Use of immersion condensers for phase contrast and brightfield techniques.

Phase contrast procedure

Step-by-step guide for setting up and performing phase contrast microscopy.

Operation of transmitted light darkfield

Darkfield setup and errors

Guides on darkfield setup and troubleshooting common errors.

Transmitted light darkfield with special darkfield condenser

Operation of oblique illumination, polarization

Assembly of polarizers

Steps for assembling and positioning polarizers and analysers.

Polarizer

Polarizer attachment and usage

Instructions for attaching filter holders and using polarizers correctly.

Crossing the polarizers

Procedure for achieving maximum extinction position when crossing polarizers.

λ- and 2/4 compensator

How to activate and use compensator plates for polarization contrast.

Operation of transmitted light interference contrast

Condenser for interference contrast

Specifies the condenser tops suitable for ICT work.

Crossing the polarizers for ICT

Steps to achieve correct polarizer crossing for optimal ICT images.

Adjustment of the condenser prisms

How to check and adjust condenser prisms for proper ICT alignment.

Objectives and Prisms for ICT

Selection of objectives and prisms suitable for ICT microscopy.

Setting ICT Contrast and Preparation

Adjusting contrast and preparing specimens for ICT imaging.

Preparation errors

Sources of error in ICT image quality

Common issues related to specimen preparation affecting ICT image quality.

Instrumentation errors affecting ICT

Lists errors related to polarizers, prisms, condenser tops, and illumination setup.

Incident light techniques

Switching on incident light

Steps for activating the incident light source and selecting the correct path.

Lamp adjustment for incident light

Guidance on adjusting the light source and reflectors for optimal imaging.

Halogen lamps: LH 106

Adjusting the collector and lamp holder

Instructions for adjusting the collector and lamp holder for LH 106 lamphousing.

Halogen, Xe and Hg lamps: LH 106 z

Adjusting light sources in LH 106 z

Guide to focusing and aligning lamp filaments/arcs in LH 106 z housing.

Mercury (Hg) and Xenon lamps (Xe) adjustment

Safety for He and Xe lamps

Collector setting

Adjusting collectors for various lamps

How to adjust the collector to achieve homogeneous illumination for different lamp types.

Linear measurements

Micrometer value and Calibration

Explains how to determine the micrometer value and calibrate measurement scales.

Care and maintenance

Main wearing and spare parts, tools

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