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Leica DM IRE2 User Manual

Leica DM IRE2
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CMN Imaging Core
January 16, 2013
1
LEICA DM IRE2 MICROSCOPE MANUAL
Neuroscience Imaging Core
Rightmire Hall
Ohio State University
Director: Tony Brown
Rightmire 060
292-1205
brown.2302@osu.edu
Facility Manager: Paula Monsma
Rightmire 062
292-3025
monsma.1@osu.edu
This manual prepared by Tony Brown.
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Leica DM IRE2 Specifications

General IconGeneral
BrandLeica
ModelDM IRE2
CategoryMicroscope
LanguageEnglish

Summary

Microscope Overview

Microscope Views

Provides views of the microscope from the left and right sides, labeling key components.

Detailed Component Views

Offers detailed views of specific components like the objective turret.

Turret Positions

Details positions for objective prism and condenser turrets and matching objectives.

Microscope Controls

Control Panel Description

Details the main control panel, including display, buttons, and indicators.

LCD Display Information

Explains the information displayed on the LCD screen and its indicators.

LCD Display Buttons

Describes the function of the buttons associated with the LCD display.

Microscope Operations and Techniques

Changing Filters

Instructions for using the motorized filter cube changer.

Changing Objectives

Guide on using objective turret controls to change magnification.

Switching Objective Modes

Procedure for switching between dry and immersion objective modes.

Adjusting Lamp Brightness

How to adjust the halogen lamp intensity.

Focusing Procedures

Explains using focusing knobs and buttons for specimen focus.

Focusing Coarseness Settings

How to change the step size for coarse and fine focusing.

Eyepieces and Slide Placement

Adjusting eyepieces and placing slides on the Z-galvo stage.

Illumination and Observation Modes

Covers transmitted light detector and bright field observation setup.

Koehler Illumination

Essential steps for obtaining good transmitted light images.

Phase Contrast Microscopy

Setup procedure for phase contrast microscopy.

Differential Interference Contrast (DIC)

Requirements and setup for DIC microscopy.

Stage Holders and Immersion Objectives

Covers switching stage holders and using immersion objectives.

Objective Maintenance

Instructions on how to clean microscope objectives.

Specimen Handling and Preparation

General tips for preparing and handling specimens.

Usage Guidelines and Support

DOs and DON'Ts for usage and when to ask for assistance.

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