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Leica RM2125RTS User Manual

Leica RM2125RTS
60 pages
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Leica RM2125 RTS
Rotary Microtome
Instructions for Use
Leica RM2125 RTS
V2.1, English - 10/2012
Order No. 14 0457 80101 RevB
Always keep this manual with the instrument.
Read carefully before working with the instrument.
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Leica RM2125RTS Specifications

General IconGeneral
TypeRotary Microtome
Section Thickness Range0.5 - 60 µm
Trimming Thickness Setting Range1 - 60 µm
Horizontal Specimen Stroke28 mm
Vertical Specimen Stroke70 mm
Specimen Clamp Rotation360°
Power Supply100-240 V, 50/60 Hz
Specimen Retraction5 - 100 µm, adjustable

Summary

Important Information

Symbols and Meanings

Explains symbols used in the manual for warnings, notes, and item numbering.

Personnel Qualification

Specifies that only trained laboratory personnel should operate the instrument.

Intended Use

Describes the Leica RM2125 RTS as a rotary microtome for specimen sectioning.

Instrument Type Identification

States that information applies to the indicated instrument type and describes identification label.

Safety

Safety Notes

Highlights the importance of reading and following safety instructions for operation and maintenance.

General Warnings

Emphasizes owner's and personnel's responsibility for safe operation and compliance with instructions.

Specific Warnings

Covers warnings related to instrument markings, transport, installation, and operation.

Integrated Safety Devices

Details safety features like handwheel lock and handwheel brake.

Knife Guard Safety

Explains the function of knife guards and safety procedures for their use.

Instrument Components and Specifications

Overview of Components

Identifies and labels key parts of the Leica RM2125 RTS from different views.

Instrument Specifications

Details technical specifications including feed systems, handwheel locking, sectioning mechanism, and trimming function.

Technical Data

Lists installation site requirements, general specifications, dimensions, weight, and optional equipment details.

Startup

Installation Site Requirements

Specifies environmental conditions (temperature, humidity, pressure) and space needed for installation.

Standard Delivery Contents

Lists items included in the basic instrument package and advises checking delivered components.

Unpacking and Installation Procedure

Provides step-by-step instructions for safely unpacking and setting up the instrument.

Inserting the Specimen Clamp

Guides the user on how to attach a specimen clamp to the specimen holder fixture.

Direct Mounting of Specimen Clamp

Explains how to directly fasten specimen clamps to the specimen holder fixture without the dovetail receptacle.

Attaching Knife Holder Base

Details the procedure for attaching the non-orientable knife holder base to the microtome.

Inserting Knife Holder

Describes how to insert a non-orientable knife holder onto the knife holder base.

Operation

Clamping the Specimen

Instructions on how to securely clamp the specimen block into the specimen clamp.

Clamping the Knife/Blade

Guidance on safely inserting and clamping the microtome knife or disposable blade.

Adjusting Clearance Angle

Explains how to set the optimal clearance angle for sectioning using index marks.

Specimen Retraction

Details the specimen retraction function, its purpose, and important precautions during use.

Orienting the Specimen

Describes how to position the specimen using a directional holder and warns against orientation during retraction.

Cutting (Trimming) Specimen

Explains how to use the coarse feed wheel for initial trimming of the specimen.

Trimming with Coarse Feed

Details trimming the specimen using the coarse feed wheel and handwheel.

Trimming with Large Section Thickness

Describes trimming by setting a large section thickness and rotating the handwheel.

Trimming with Mechanical Function

Explains how to use the mechanical trimming function with its specific stages.

Sectioning Procedure

Guides on setting section thickness and rotating the handwheel for consistent sectioning.

Changing Specimens

Provides steps for safely removing and replacing the specimen in the clamp.

Standard Specimen Clamp

Describes the standard specimen clamp for rectangular blocks and how to mount specimens.

Vee Insert for Round Specimens

Explains how to use the Vee insert to clamp round specimens in the standard clamp.

Foil Clamp Type 1

Details how to use Foil Clamp Type 1 for small foil pieces and flat, angular specimens.

Foil Clamp Type 2

Describes how to use Foil Clamp Type 2 for large foil ribbons.

Universal Cassette Clamp

Explains how to mount and clamp cassettes in the Universal Cassette Clamp (UCC).

Round Specimen Holder

Details how to use the round specimen holder with inserts and clamping rings for cylindrical specimens.

Knife Holder Base (Non-Orientable)

Explains how to move and secure the non-orientable knife holder base.

Knife Holder Base (Lateral Movement)

Describes how to move and secure the knife holder base with lateral movement.

Knife Holder N/NZ

Introduces Knife Holders N and NZ for various knives and their height adjustment feature.

Inserting the Knife

Provides detailed steps for inserting a knife into the holder.

Knife Height Adjustment

Explains how to adjust the knife height using lay-on edges as reference.

Lateral Repositioning of the Knife

Details how to reposition the knife laterally on the holder base.

Knife Holder E/E-TC

Describes Knife Holder E-TC for tungsten carbide blades and its blade insertion process.

Knife Holder E

Introduces Knife Holder E for disposable blades and its insertion/changing procedure.

Changing the Rear Pressure Plate

Explains how to remove and replace the rear pressure plate on Knife Holder E.

Adjusting the Rear Pressure Plate

Details the procedure for height and parallel adjustment of the rear pressure plate.

Adjusting the Front Pressure Plate

Explains how to adjust the height and clearance angle of the front pressure plate.

Overview of Accessories

Provides a visual overview of available accessories for the microtome.

Cleaning and Maintenance

Cleaning the Instrument

Outlines general safety precautions and steps for cleaning the microtome's exterior surfaces.

Knife Holder E Cleaning

Details the disassembly and cleaning procedure for Knife Holder E, including pressure plate removal.

Universal Cassette Clamp Cleaning

Explains the cleaning process for the universal cassette clamp, including paraffin removal.

Maintenance Instructions

Recommends regular checks, cleaning, and lubrication for trouble-free operation.

Optional accessories

Knife Holder Bases and Holders

Covers knife holder bases (non-orientable, lateral movement) and Knife Holder N.

Knife Holders NZ and E

Details Knife Holders NZ and E for various blades and their features.

Knife Holders E (High-Profile) and E-TC

Details Knife Holder E for high-profile blades and Knife Holder E-TC for carbide blades.

Knife Holder Pressure Plates

Lists pressure plates for different blade types.

Water Bath Assembly and Disposable Blades

Includes water bath assembly and disposable blades (low/high profile).

Disposable Blades TC-65 and Steel Knives

Lists TC-65 blades and 16/12 cm steel knives with profile c.

Steel and Carbide Knives

Lists 16/12 cm steel knives (profile d) and carbide knives.

Knife Case

Describes the variable knife case.

Specimen Holder Fixture and Clamps

Covers non-orientable fixture, standard clamp, and vee insert.

Universal Cassette Clamp and Foil Clamps

Details universal cassette clamp and foil clamps (Type I, Type II).

Round Specimen Holders and Clamping Rings

Describes round specimen holders and various sizes of clamping rings.

Clamping Rings and Section Waste Tray

Lists clamping rings (15mm, 25mm) and section waste tray.

Dust Cover and Safety Gloves

Covers dust cover and safety gloves (sizes S and M).

Troubleshooting

Thick/Thin Sections

Addresses issues like alternating thick/thin sections, chattering, or torn specimens.

Compressed Sections

Deals with sections being compressed, folded, or squeezed together.

Sectioning Issues

Covers "stripes" in sections, noises during sectioning, and high blade consumption.

Instrument Malfunctions

Addresses issues like no feed motion and high blade consumption.

Warranty and Service

Warranty Information

Outlines the manufacturer's guarantee and warranty terms.

Service Information

Provides contact details and required information for technical support or spare parts.

Decommissioning and Disposal

Advises on proper disposal of the instrument according to local regulations.

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