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Leica CM1520 User Manual

Leica CM1520
100 pages
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Leica CM1520
Cryostat
Instructions for Use
English
Order No.: 14 0491 81101 - Revision K
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.0, Revision K - 10.2019

Table of Contents

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Leica CM1520 Specifications

General IconGeneral
BrandLeica
ModelCM1520
CategoryLaboratory Equipment
LanguageEnglish

Summary

Important Information

Symbols used in the text and their meaning

Explanation of various symbols and their significance within the manual for user understanding.

Qualification of personnel

Specifies the required qualifications and training for operating personnel.

Intended use of the instrument

Defines the designated applications and scope for the Leica CM1520 cryostat.

Instrument type

Details on the specific model and its applicability within the manual.

Safety

Safety Notes

General safety precautions and warnings to be observed during operation.

Hazard warnings

Highlights potential hazards and risks associated with the instrument's use.

Safety devices

Description of built-in safety features like handwheel lock and safety guard.

Operating Conditions

Specifies environmental and spatial requirements for safe instrument operation.

Operating the instrument

Guidelines and warnings for safe operation of the cryostat.

Cleaning and disinfection

Procedures for cleaning and disinfecting the instrument safely.

Removing the microtome

Information regarding the microtome's encapsulation and user removal.

Maintenance

General instructions for maintaining the instrument's safe operation.

Technical Data

Equipment identification

Details on the instrument's model and identification numbers.

Electrical specifications

Information on voltage, frequency, and power requirements.

Dimensions and weight specification

Physical dimensions and weight of the instrument and its packaging.

Environmental specification

Operating and storage environmental parameters for the cryostat.

Chamber cooling

Technical details regarding the cryochamber's cooling system.

Microtome

Specifications related to the integrated microtome component.

Installing the Instrument

Installation site requirements

Criteria for selecting a suitable installation location for the cryostat.

Unpacking instructions for the Leica CM1520 cryostat

Step-by-step guide for safely unpacking the instrument.

Transport to the site

Instructions for safely moving the instrument to its final location.

Standard delivery - packing list

List of components included in the standard delivery package.

Installing the handwheel

Procedure for attaching the handwheel to the instrument.

Instrument Setup

Electrical connection

Guidelines for safely connecting the instrument to the power supply.

Preparations for starting up the instrument

Steps to perform before initial startup of the cryostat.

General overview of the Leica CM1520

Identification of major components and parts of the cryostat.

Power switch and circuit breaker

Location and function of the main power switch and circuit breaker.

Switching on the instrument

Procedure for powering on the instrument after setup.

Operating the Instrument

Control panel 1

Description of functions and buttons on the primary control panel.

Configuring the desired values

How to set and adjust instrument parameters like time and temperature.

Setting the automatic defrost time (cryochamber)

Procedure for programming the automated defrost cycle time.

Programming the temperature of the cryochamber

Steps to set the target temperature for the cryochamber.

Setting the section thickness

How to select and adjust the desired section thickness.

Display lock

Functionality for locking the control panel display to prevent accidental changes.

Control panel 2 – Electric coarse feed

Operation of the electric coarse feed controls for specimen movement.

Daily Use of the Instrument

The sectioning process

Detailed steps for preparing and performing the sectioning procedure on the cryostat.

Specimen freezing

Instructions for freezing specimens, including temperature selection and methods.

Specimen discs

How to use and insert specimen discs into the specimen head for sectioning.

Sectioning

Procedures for inserting blade/knife holders and adjusting sectioning parameters.

Clearance angle adjustment

Guidance on setting the optimal clearance angle for different specimen types.

Blade holder CE

Detailed instructions for inserting blades into the CE blade holder.

Adjusting the anti-roll guide system

How to adjust the anti-roll guide for optimal section collection.

Knife holder CN

Procedures for inserting and removing knives using the CN knife holder.

Temperature selection chart (in minus °C)

A chart recommending optimal chamber temperatures for various tissue types.

Defrosting

Information on cryochamber and quick freeze shelf defrosting procedures.

Ending work

Steps to follow to properly finish the daily routine and end work.

Troubleshooting

Error messages in the display

List of error codes, their causes, and recommended remedies for display issues.

Temperature control button

Explanation of the temperature control button and its function.

Possible sources of error, causes and remedies

Comprehensive guide to diagnosing and resolving common operational problems.

Cleaning, Disinfection, Maintenance

Cleaning

Procedures for routine cleaning of the cryostat and its components.

Disinfection at room temperature

Steps for disinfecting the cryochamber and associated parts.

Maintenance

General instructions and recommendations for instrument upkeep.

Replacing the fuses

Instructions for safely replacing the instrument's fuses.

Removing the sliding window

Procedure for removing and reinstalling the sliding window.

Ordering Information, Components and Consumables

Ordering information

Catalog numbers and descriptions for various instrument parts and accessories.

Components and consumables

Listing of additional components and consumable items for the cryostat.

Consumables

Details on disposable items and supplies for the instrument.

Mobile heat extractor - application

How to use the mobile heat extractor to accelerate specimen freezing.

Cold extraction - using the Thermal block

Procedure for using the thermal block to remove frozen specimens.

Stationary heat extractor (optional) - application

Instructions for installing and using the optional stationary heat extractor.

Installing the shelf (movable) (optional)

Guide for installing the optional movable shelf.

Warranty and Service

Warranty

Information regarding the product warranty and its terms.

Service information

Contact details and procedures for obtaining technical support or spare parts.

Decontamination Certificate

Decontamination Certificate

Requirements and procedures for product decontamination prior to return or service.

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