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Leica CM1510 User Manual

Leica CM1510
32 pages
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Instruction Manual
Leica CM1510 V2.1 English – 12/2001
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica CM 1510
Cryostat
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Leica CM1510 Specifications

General IconGeneral
BrandLeica
ModelCM1510
CategoryLaboratory Equipment
LanguageEnglish

Summary

Important Information

Safety Instructions for Handling the Instrument

Handwheel Lock and Knife Guard Safety

Safe operation procedures for the handwheel lock and knife guard.

General Operational Hazards

Highlights risks like knife injury, frostbite, and handling hazardous materials.

Transport, Installation, and Power Safety

Guidelines for safe setup, power connection, and environmental conditions.

Sectioning Safety Precautions

Safe practices during sectioning, including knife handling and specimen manipulation.

Technical Data

General Description

Leica CM 1510 Components Overview

Diagram and list of the main components of the Leica CM 1510 cryostat.

Product Features and Delivery Range

Overview of the cryostat's capabilities and items included in the standard package.

Optional Accessories Information

Details on available optional accessories for enhancing cryostat functionality.

Unpacking and Installation

Site Requirements and Transport Safety

Criteria for location selection and safe instrument transport.

Handwheel Assembly

Step-by-step guide for assembling the handwheel onto the cryostat.

Setup and Operation

Mains Power Connection and Pre-Operation Checks

Guidelines for electrical connection and essential preparation before use.

Switches, Controls, and Temperature Settings

Explanation of instrument controls, panel functions, and temperature selection.

Temperature Selection Chart for Tissues

Guide for setting appropriate cryochamber temperatures based on tissue type.

Daily Operation

Specimen Freezing and Mounting

Procedure for cutting, freezing, and mounting specimens for sectioning.

Knife/Blade Handling and Sectioning

Safe insertion of blades, specimen movement, trimming, and sectioning.

Section Transfer and Defrosting

Transferring sections to slides and managing defrost cycles.

Troubleshooting

Sectioning and Flattening Issues

Troubleshooting common problems like smeared, splintered, or improperly flattened sections.

Advanced Sectioning and Condensation Problems

Solutions for section chatter, condensation, plate damage, and thick-thin sections.

Operational and Refrigeration Faults

Resolving issues with specimen adhesion, feed, refrigeration, and unit operation.

Cleaning and Disinfection

Routine Cleaning and Disinfection

Steps for cleaning the cryochamber and using disinfectant.

Microtome Removal and Reinstallation

Procedures for safely removing and reinstalling the microtome for cleaning.

Maintenance

General Maintenance and Servicing

Routine checks, cleaning, and recommended service intervals for the instrument.

Fuse and Lamp Replacement

Guides for replacing fuses, resetting cutouts, and changing the lamp.

Optional Accessories

Heat Extractors for Freezing

Description and use of heat extractors for accelerated specimen freezing.

Thermal Block for Specimen Removal

How to use the thermal block to easily remove frozen specimens from discs.

Appendix

Product Changes, Warranty, and Disposal

Information on product updates, warranty, and proper disposal methods.

Technical Service and Support

Guidelines for contacting technical support and requesting service or parts.

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