What to do if Leica Laboratory Equipment is inoperational?
If the cryostat is inoperational, check the mains plug connection and reconnect the automatic cut-out (switch the instrument back on). The causes may be: mains plug not properly connected or automatic cut-out has been triggered.
What to do if sections smear when using Leica CM3050S Laboratory Equipment?
If sections smear, it may be because the specimen isn't cold enough or the knife and/or anti-roll plate haven't reached a low enough temperature, causing the sections to melt. To fix this, select a lower temperature and ensure the knife and anti-roll plate reach the chamber temperature.
What to do if sections tear when using Leica CM3050S Laboratory Equipment?
If sections tear, it could be due to: * The temperature being too low for the tissue. * A blunt, dirty, dusty, frosted, or rusty knife. * A damaged upper edge of the anti-roll plate. * Hard particles in the tissue. * A dirty rear face of the knife. To resolve this: 1. Select a higher temperature and wait for it to be reached. 2. Address the knife issues by cleaning, rotating, or exchanging it. 3. Rotate or exchange the anti-roll plate if its upper edge is damaged. 4. If possible, section at a lower plane if hard particles are present. 5. Clean the rear face of the knife.
What causes sections to splinter in Leica CM3050S?
Sections may splinter or not flatten properly due to: * The specimen being too cold. * Static electricity or draft. * The specimen not being cold enough. * A large surface specimen. * A poorly adjusted anti-roll plate. * A poorly aligned anti-roll plate relative to the knife edge. * An incorrect clearance angle. * A blunt or damaged knife. To solve this: 1. Select a higher temperature if the specimen is too cold. 2. Eliminate static electricity or draft. 3. Select a lower temperature if the specimen isn't cold enough. 4. Trim the specimen parallel and increase section thickness for large surface specimens. 5. Readjust the anti-roll plate and align it correctly. 6. Set the correct clearance angle. 7. Use a different part of the knife or replace it.
What to do if sections are not flattened despite correct temperature and anti-roll plate alignment in Leica CM3050S Laboratory Equipment?
If sections still aren't flattening correctly, even with the right temperature and anti-roll plate alignment, the issue might be: * A dirty knife or anti-roll plate. * A damaged edge of the anti-roll plate. * A blunt knife. To fix this: 1. Clean the knife and anti-roll plate with a dry cloth or brush. 2. Replace the anti-roll plate if its edge is damaged. 3. Use a different part of the knife or replace it.
Why do sections curl on the anti-roll plate of Leica CM3050S Laboratory Equipment?
If sections curl on the anti-roll plate, it's likely because the anti-roll plate doesn't protrude far enough beyond the knife edge. To fix this, readjust the anti-roll plate correctly.
What causes chatter marks during sectioning with Leica Laboratory Equipment?
Chatter marks during sectioning can be caused by several factors: * The specimen is insufficiently frozen onto the specimen disc. * The specimen disc isn't clamped tightly enough. * The specimen holder ball joint isn't clamped. * The knife isn't clamped tightly enough. * The selected section thickness is too thick, causing the specimen to detach. * The specimen is very hard and inhomogeneous. * The knife is blunt. * The knife profile is inappropriate for the specimen type. * The clearance angle is incorrect. To address this: 1. Refreeze the specimen onto the disc. 2. Check the disc clamping and ball joint clamping. 3. Check the knife clamping. 4. Increase section thickness or reduce specimen surface area. 5. Use a different part of the knife or replace it. 6. Use...
What to do if the sectioning motor is inoperational in Leica CM3050S?
If the sectioning motor is inoperational, connect the footswitch and reconnect the automatic cut-out by pushing it inwards until it locks in place. The causes may be: footswitch not connected or automatic cut-out of sectioning motor has been triggered.
What to do if the coarse feed plug is not reconnected properly in Leica CM3050S?
If the coarse feed plug has not been reconnected properly after reinstallation of the microtome, reconnect the coarse feed plug properly.
How to fix inconsistent specimen feed in Leica CM3050S?
Inconsistent or insufficient specimen feed may occur if the microtome wasn't completely dry when refrigeration was switched on, leading to ice build-up inside the micrometer mechanism, or if the microtome is defective. To resolve this, remove the microtome from the chamber and dry it, possibly with a hairdryer (used only outside the cryochamber!) or at ambient temperature. If the problem persists, contact Technical Service.
Emphasizes observing all safety and caution notes throughout the chapter.
Provides instructions on how to lock the handwheel for safe operation.
Safety precautions for transporting and installing the cryostat.
Explains the purpose of each button on Control Panel 1.
Describes the information displayed on Control Panel 1.
Lists and explains error messages indicating instrument malfunctions.
Emphasizes observing all safety and caution notes throughout the chapter.
Provides instructions on how to lock the handwheel for safe operation.
Safety precautions for transporting and installing the cryostat.
Explains the purpose of each button on Control Panel 1.
Describes the information displayed on Control Panel 1.
Lists and explains error messages indicating instrument malfunctions.
General| Specimen Cooling | Yes |
|---|---|
| Motorized Sectioning | Yes |
| Vertical Specimen Stroke | 60 mm |
| Microtome Type | Rotary microtome |
| Power Supply | 100-240 V, 50/60 Hz |
| Specimen Retraction | Automatic |
| Specimen Holder | Universal specimen holder |
| Section Thickness Range | 0.5 µm to 100 µm |
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