Leica CM3050S User Manual (English)

Questions and Answers:

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Douglas SmithAug 21, 2025
What to do if frost forms on chamber walls and microtome of Leica Laboratory Equipment?
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  Amanda SullivanAug 21, 2025
  If you notice frost on the chamber walls and microtome, it could be due to a few reasons: * The cryostat is exposed to draft from open windows, doors, or air conditioning. * The sliding window has been open for too long at a very low chamber temperature. * Frost build-up is caused by breathing into the cryochamber. To resolve this: * Eliminate draft sources or change the location of the cryostat. * Close the sliding window. * Consider wearing a mask.
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Sarah KramerAug 22, 2025
What to do if sections smear when using Leica CM3050S Laboratory Equipment?
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  gcastilloAug 22, 2025
  If sections smear, it may be because the specimen isn't cold enough or the knife and/or anti-roll plate haven't reached a low enough temperature, causing the sections to melt. To fix this, select a lower temperature and ensure the knife and anti-roll plate reach the chamber temperature.
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Daniel GillespieAug 26, 2025
What to do if sections tear when using Leica CM3050S Laboratory Equipment?
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  Renee PowellAug 26, 2025
  If sections tear, it could be due to: * The temperature being too low for the tissue. * A blunt, dirty, dusty, frosted, or rusty knife. * A damaged upper edge of the anti-roll plate. * Hard particles in the tissue. * A dirty rear face of the knife. To resolve this: 1. Select a higher temperature and wait for it to be reached. 2. Address the knife issues by cleaning, rotating, or exchanging it. 3. Rotate or exchange the anti-roll plate if its upper edge is damaged. 4. If possible, section at a lower plane if hard particles are present. 5. Clean the rear face of the knife.
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Darrell PatrickAug 27, 2025
What causes sections to splinter in Leica CM3050S?
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  joshua32Aug 28, 2025
  Sections may splinter or not flatten properly due to: * The specimen being too cold. * Static electricity or draft. * The specimen not being cold enough. * A large surface specimen. * A poorly adjusted anti-roll plate. * A poorly aligned anti-roll plate relative to the knife edge. * An incorrect clearance angle. * A blunt or damaged knife. To solve this: 1. Select a higher temperature if the specimen is too cold. 2. Eliminate static electricity or draft. 3. Select a lower temperature if the specimen isn't cold enough. 4. Trim the specimen parallel and increase section thickness for large surface specimens. 5. Readjust the anti-roll plate and align it correctly. 6. Set the correct clearance angle. 7. Use a different part of the knife or replace it.
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angelmartinezAug 29, 2025
What to do if sections are not flattened despite correct temperature and anti-roll plate alignment in Leica CM3050S Laboratory Equipment?
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  Cheryl PrattAug 29, 2025
  If sections still aren't flattening correctly, even with the right temperature and anti-roll plate alignment, the issue might be: * A dirty knife or anti-roll plate. * A damaged edge of the anti-roll plate. * A blunt knife. To fix this: 1. Clean the knife and anti-roll plate with a dry cloth or brush. 2. Replace the anti-roll plate if its edge is damaged. 3. Use a different part of the knife or replace it.
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Samantha RodriguezAug 31, 2025
Why do sections curl on the anti-roll plate of Leica CM3050S Laboratory Equipment?
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  Cindy MillerAug 31, 2025
  If sections curl on the anti-roll plate, it's likely because the anti-roll plate doesn't protrude far enough beyond the knife edge. To fix this, readjust the anti-roll plate correctly.
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Connie SmallSep 2, 2025
What causes scraping noise during sectioning with Leica CM3050S Laboratory Equipment?
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  Dale ReevesSep 2, 2025
  A scraping noise during sectioning and specimen return stroke indicates that the anti-roll plate protrudes too far beyond the knife edge and is scraping against the specimen. Readjust it correctly to resolve this.
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wolfetoniSep 3, 2025
What causes undulated sections in Leica CM3050S Laboratory Equipment?
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  Michael LarsonSep 3, 2025
  Undulated sections can be caused by a damaged knife or a damaged edge of the anti-roll plate. To resolve this, use a different part of the knife or replace it, or replace the anti-roll plate.
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xmooreSep 5, 2025
Why does condensation form on the anti-roll plate and knife during cleaning of Leica CM3050S Laboratory Equipment?
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  Jessica ZimmermanSep 5, 2025
  Condensation forms on the anti-roll plate and knife during cleaning because the brush, forceps, cloth, or other cleaning item is too warm. Make sure to use pre-cooled accessories and cleaning materials, storing them on storage shelves inside the cryochamber.
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Jared Rios Jr.Sep 7, 2025
How to fix inconsistent specimen feed in Leica CM3050S?
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  Alan RiosSep 7, 2025
  Inconsistent or insufficient specimen feed may occur if the microtome wasn't completely dry when refrigeration was switched on, leading to ice build-up inside the micrometer mechanism, or if the microtome is defective. To resolve this, remove the microtome from the chamber and dry it, possibly with a hairdryer (used only outside the cryochamber!) or at ambient temperature. If the problem persists, contact Technical Service.

Leica CM3050S Specifications

General

Specimen Cooling	Yes
Motorized Sectioning	Yes
Vertical Specimen Stroke	60 mm
Microtome Type	Rotary microtome
Power Supply	100-240 V, 50/60 Hz
Specimen Retraction	Automatic
Specimen Holder	Universal specimen holder
Section Thickness Range	0.5 µm to 100 µm