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General| Brand | Leica |
|---|---|
| Model | Stellaris 5 |
| Category | Microscope |
| Language | English |
Steps for powering on the computer, logging in, and activating the scanner box.
Starting the LAS X software and selecting the microscope configuration for use.
Settings on the TFT screen for viewing samples using incident light (fluorescent).
Settings on the TFT screen for viewing samples using transmitted light (brightfield).
Selecting the correct objective and immersion medium, setting port and magnification.
Modifying the intensity for transmitted (TL) or incident (IL) light using software controls.
Exploring the Configuration tab for system settings, lasers, and hardware options.
Introduction to the Acquire tab for setting up imaging parameters and starting scans.
Understanding different acquisition modes (2D, 3D, time-lapse) and key parameters.
Navigating the image compass and using Live/Fast Live modes for sample observation.
Configuring detectors and initiating the imaging acquisition process.
Using the Dye Assistant for automated channel setup based on selected fluorophores.
Detailed steps for setting up imaging channels using the Image Compass interface.
Step-by-step guide for manual channel setup, including detector and laser adjustments.
Fine-tuning channels, adjusting laser wavelengths, and utilizing the "Add Laser" feature.
Accessing integrated help features and tutorials within the LAS X software.
Adjusting detector gain and laser power to manage pixel intensity and avoid overexposure.
Settings for pixel size, scanning speed, and bidirectional scanning for image quality.
Techniques for averaging images to reduce noise and adjusting the pinhole for optical sectioning.
Configuring Z-stacks, including step size, number of slices, and optimization.
Utilizing the Lightning module for on-the-fly deconvolution to enhance resolution.
Using tools like Over-/Underexposure LUT, Auto contrast, Scalebar, and Zoom.
Managing channel visibility, viewing histograms, and accessing the 3D viewer.
Capturing snapshot views, including MIP and orthogonal views for analysis.
Saving projects, understanding .lif and RAW formats, and managing image datasets.
Exporting image files and reusing previous imaging settings for new scans.
Steps for cleaning, logging, saving, and powering down the microscope and computer.
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