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Leica DMI Series User Manual

Leica DMI Series
356 pages
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Leica DMI3000B,
DMI4000B, DMI6000B
Instructions · Bedienungsanleitung · Mode d’emploi

Table of Contents

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Leica DMI Series Specifications

General IconGeneral
Microscope TypeInverted Microscope
ObjectivesAchromatic, Plan Achromatic
Optical SystemInfinity Optical System
IlluminationLED or halogen transmitted light
Contrast MethodsBrightfield, Phase Contrast, DIC, Fluorescence
Observation TubesBinocular, Trinocular
FocusingCoarse and Fine focusing
StageMotorized stage
Camera PortIntegrated camera port for digital imaging
CondenserVarious condensers available for different contrast methods (e.g., Brightfield condenser, Phase contrast condenser, DIC condenser)
ImagingDigital imaging capabilities when used with a compatible microscope camera and software

Summary

Important Notes About this Manual

Text symbols, pictograms and their meanings

Explains the various text symbols and pictograms used throughout the manual.

Safety Notes

General Safety Notes

Provides general safety guidelines, compliance standards, and warnings for instrument operation.

Electrical Safety

Details electrical specifications and safety precautions for microscope components and accessories.

Transport and storage

Instructions for safe transport and storage conditions, including temperature and humidity limits.

Safety Instructions for Handling the Light Sources

Safety precautions for handling light sources, addressing irradiation risks and proper operation.

Notes on handling laser devices

Information regarding the unsuitability for laser coupling and alternative options.

Notes on handling immersion oil

Safety advice for handling immersion oil, emphasizing skin contact avoidance and data sheets.

Safety Instructions for Handling Acids and Bases

Emphasizes caution when handling acids and aggressive chemicals to prevent contact.

Disposal

Guidelines on proper disposal of the product according to national and EU regulations.

Type labels

Illustrates and describes the type labels found on the DMI3000B, DMI4000B, and DMI6000B models.

Overview of the Leica DMI Series

Contrast Methods

Details the various contrast methods supported by the DMI Series microscopes.

Transmitted Light Axis

Describes the features and capabilities of the transmitted light illumination arm.

Incident Light Axis

Outlines the features of the incident light axis for different DMI models.

Tube

Describes the design and adjustment features of the microscope tubes.

Magnification Changer

Details the manual and motorized magnification changer options.

Objective Turret

Describes the motorized and manual objective turrets for different DMI models.

Stages

Lists various types of specimen stages available for the DMI Series.

Condensers

Details the features of condensers for DMI4000 B and DMI6000 B, including housings and heads.

Z Focus

Describes the motorized and manual Z focus systems for different DMI models.

Observation Ports

Lists and describes the available observation ports for DMI6000 B and DMI4000 B.

Controls

Details the control buttons, hand wheels, and remote modules available for DMI series.

Electronics Box

Explains the function of the electronics boxes and their connections.

Interfaces

Lists the available interface ports like USB and RS232 for data communication.

Software Tools

Mentions the Leica Application Suite (LAS) for microscope and camera control.

Unpacking the Microscope

Installation Location

Specifies requirements for the installation environment and ambient conditions for optimal operation.

Transport

Details components to disassemble for safe transportation and packaging to prevent vibration damage.

Weight

States the approximate weight of the fully equipped microscope for handling purposes.

Assembly

Assembly Tools

Lists the tools required for assembling the microscope, often provided with the unit.

Installation of the Transmitted Light Illumination Carrier (TL)

Step-by-step guide for installing the transmitted light illumination carrier onto the microscope.

Installation of the DIC Module and DIC Objective Prisms

Instructions for installing the DIC module and objective prisms, including prism disk replacement.

Installation of Specimen Stages

Describes the various types of specimen stages and their installation procedures.

Installation of Condensers

Details the installation of condensers, light rings, and slit diaphragms into the condenser disk.

Installation of Eyepieces

Instructions for inserting eyepieces into the eyepiece tubes and adjusting for correct magnification.

Installation of Objectives

Guidance on installing objectives into the objective turret and checking parfocality.

Installation of Filters in the Illumination Arm

How to install and change filters in the illumination arm's filter magazine.

Installing the transmitted Light Lamp Housing

Steps to mount and connect the transmitted light lamp housing to the microscope.

Installation and Replacement of the transmitted Light Lamps

Procedures for changing halogen lamps in the 107 or 107/2 lamp housings safely.

Installing the Lamp Housing Mount and Mirror Housing

Instructions for mounting the lamp housing or mirror housing onto the rear wall of the stand.

Installation and Replacement of Incident Light Lamps

Safety precautions and procedures for installing incident light lamps, including cooling time.

Equipping the Incident Light Turret Disk

Guide on how to equip the incident light turret disk with filter cubes for fluorescence.

Inserting the Front Module Slider

Instructions for inserting the front module slider for integrated contrast methods.

Installation of the Polarizer and Analyzer

Steps for installing the polarizer and analyzer components for polarization contrast.

Optional Accessories

Information on connecting cameras and other optional accessories to the microscope.

Connection to the Electronics Box

Details on connecting the microscope to various CTR electronics boxes via specific ports.

Connection to the Computer

How to connect the microscope to a computer using serial or USB cables.

Connection to the Power Supply

Instructions for connecting the electronics box and external supply units to AC power.

Start-up

Functional Principle (Leica DMI4000 B and Leica DMI6000 B)

Explains the intelligent automation and control elements of the DMI4000 B and DMI6000 B.

Switching on the Microscope

Step-by-step guide for powering on the microscope and its components correctly.

The LeicaDisplay

Describes the microscope's current settings display and its areas/lines.

The Function Buttons on the Stand

Explains the fixed and variable function buttons on the microscope stand for operation.

Function Buttons on the Front Panel

Details the functions of buttons located on the front control panel of the microscope.

The SmartMove Remote Control Module

Explains the functions of the SmartMove remote control module for stage and focus control.

Transmitted light

Guides on setting up transmitted light illumination, including Koehler illumination preparation.

Checking Phase Contrast Rings

How to check and center phase contrast rings for different objectives.

Checking modulation contrast slit diaphragms

Verifies the positioning and seating of slit diaphragms for modulation contrast.

Setting the Motorized Polarizer

Instructions for setting the motorized polarizer to achieve optimal darkening.

Adjusting the Light Sources

Procedures for adjusting transmitted and incident light sources for optimal illumination.

Operation

Switching on

Guide for powering on the microscope and its components correctly.

Contrast Methods

Overview of contrast methods available on DMI4000B/DMI6000B and DMI3000B.

Bright Field (TL)

Steps for setting up bright field transmitted light illumination.

Phase Contrast (TL)

Instructions for setting up phase contrast transmitted light illumination.

Dark Field (TL)

Procedures for dark field transmitted light illumination setup.

Polarization (TL)

Guide for setting up transmitted light polarization contrast method.

Differential Interference Contrast (TL)

Steps for setting up transmitted light Differential Interference Contrast (DIC).

Integrated Phase Contrast (TL)

Instructions for integrated phase contrast transmitted light setup.

Integrated Modulation Contrast (TL)

Guide for setting up integrated modulation contrast transmitted light.

Fluorescence

Procedures for operating the fluorescence module and changing filter cubes.

Combination Methods

Details how to use combination methods like FLUO/PH and FLUO/DIC.

Focusing

Explains how to focus the image and set focus stops using various controls.

Tubes

Information on adjusting viewing distance, angle, and beam splitting in photo tubes.

Port selection

How to select active ports (VIS, Left, eyepieces) on different DMI models.

Eyepieces

Instructions for using eyepieces, including reticle focus and vision correction.

Objectives

Guidance on changing objectives and automatic settings selected by the microscope.

Stages and Object Displacement

How to control stage movement, set increments, and store positions.

Magnification Changer

Details manual and motorized magnification changers and how to use them.

Light sources

How to adjust light intensity for transmitted and fluorescence illumination.

Aperture and Field Diaphragm

Instructions for adjusting aperture and field diaphragms on DMI models.

Trouble Shooting

Stand

Troubleshooting common problems related to the microscope stand not responding.

Illumination

Troubleshooting issues with the microscope illumination system.

Bright field

Solutions for problems in bright field mode, like focus or illumination issues.

Dark Field

Troubleshooting steps for dark field contrast issues.

Phase contrast

Solutions for problems preventing phase contrast observation.

Polarization

Troubleshooting steps for polarization contrast issues.

Transmitted light interference contrast

Solutions for issues with transmitted light interference contrast.

Fluorescence

Troubleshooting fluorescence imaging problems like dark or weak images.

LeicaScreen

Addresses troubleshooting for 'Init Error' messages on the LeicaScreen.

Care of the Microscope

Dust Cover

Guidance on using the dust cover to protect the microscope from dust after use.

Cleaning

Procedures for cleaning coated parts, the microscope stage, and optics.

Cleaning Glass Surfaces and Objectives

Instructions for cleaning glass surfaces and microscope objectives.

Removing Immersion Oil

Steps for safely removing immersion oil from components using cloths and alcohol.

Handling Acids and Bases

Safety precautions for handling acids and aggressive chemicals.

Major Consumable and Replacement Parts

Dimensions

Space requirements

Information on the physical space requirements for the microscope setup.

Height compensation plate*

Details the purpose and use of the height compensation plate for adjusting viewing height.

Abbreviations and Pictograms

EU Declaration of Conformity

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