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Leica DMIRE2 User Manual

Leica DMIRE2
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1
Cell Imaging Unit – UIC
LEICA DMIRE2 MICROSCOPE MANUAL
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Leica DMIRE2 Specifications

General IconGeneral
BrandLeica
ModelDMIRE2
CategoryMicroscope
LanguageEnglish

Summary

Anatomy of the Leica DMIRE2 Microscope

Initialisation Procedure

Left Side of Microscope View

Halogen Lamp and Focus Controls

Details halogen lamp intensity adjustment wheel and coaxial coarse & fine focus controls.

Epifluorescence and Filter Controls

Covers illumination/field diaphragms, objective prism turret, and filter cube turret.

DMIRE2 Front Panel Overview

Microscope Status Display

Alpha-numeric display shows objective position, step size, limits, objective settings, filter cube, and mode.

Control Buttons for Display

Buttons for learn mode, voltage/objective readout, and setting focus limits/step size.

Fluorescence Filter Cube Indicators

LED panel indicates active fluorescence filter cube status and associated fluorochromes.

Filter Cube Selection Switches

Two-way switches to rotate the filter cube turret; active cube is illuminated red.

Mercury Lamp Shutter Control

One-way switch; red LED indicates shutter is closed.

Port Selection Switch

Two-way switch directs light to eyepieces, scanner, or disables output.

Contrast Methods

Brightfield Illumination

Adjust halogen lamp brightness using the wheel on the front left.

Fluorescence Illumination

Switch off transmitted light, switch on mercury lamp, cycle cubes via switches.

Magnification and Objective Handling

Objective Lens Specifications

Details objective types, magnification, NA, immersion, cover-glass, DIC prisms, and pixel size.

Changing Objective Lens

Use control panel buttons to switch immersion modes; use turret buttons to change objectives.

Focusing Mechanism

Coaxial Focus Knobs

Knobs on left/right side for up/down focusing.

Electronic Focussing Buttons

Buttons on right side for up/down focusing, respecting set limits.

Scanning Mode Operation

Acquiring Images with Image-Pro Plus

Start software, use camera icon, adjust exposure, and snap images.

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