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Leica DM1000 User Manual

Leica DM1000
178 pages
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Leica DM1000
Leica DM1000 LED
Operating Manual · Bedienungsanleitung · Mode d’emploi

Table of Contents

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Leica DM1000 Specifications

General IconGeneral
Microscope TypeUpright Microscope
FocusCoaxial coarse and fine focus knobs
ApplicationResearch
IlluminationLED
Eyepiece10x
StageMechanical stage
CondenserAbbe condenser
Optical SystemInfinity optical system
Magnification Range40x to 1000x
Nosepiece4- or 5-position nosepiece

Summary

Important Notes about this Manual

Text symbols, pictograms and their meanings

Explains the meaning of symbols, pictograms, and text conventions used in the manual.

Intended Purpose of the Microscope

Safety Notes

General Safety Notes

Provides general safety guidelines and compliance information for the microscope.

Electrical Safety

Details electrical specifications and safety precautions for microscope operation and power supply.

Disposal

Provides instructions and regulations for the proper disposal of the microscope and accessories.

Overview of the Instrument

Unpacking the Microscope

Installation Location

Specifies the recommended environmental conditions and location for microscope setup and operation.

Transport

Outlines procedures for safely disassembling and packaging the microscope for transport.

Assembling the Microscope

Stage

Details the steps for attaching and securing the microscope stage and specimen holder.

Adjusting the Focus Stop

Explains how to adjust the focus stop to prevent objective collision and set focal planes.

Stage Lock*

Describes the procedure for mounting and using the stage lock mechanism.

Condenser

Guides on how to properly insert and center the microscope condenser.

Tube and Eyepieces

Instructions for mounting the tube and inserting eyepieces, including notes for fluorescence applications.

Objectives

Provides guidelines for selecting, mounting, and arranging objectives on the nosepiece.

Light Source for the Transmitted Light Axis

Details the process of replacing the tungsten halogen lamp in the transmitted light illumination system.

Fluorescence Illuminator*

Explains the mounting procedure for the fluorescence illuminator before the tube.

106z Lamp Housing*

Provides safety notes and instructions for inserting gas discharge lamps into the 106z lamp housing.

Analyzer and Polarizer*

Describes the installation of the analyzer and polarizer components for contrast methods.

Lambda Plate Compensator*

Details the steps for attaching the lambda plate compensator to the microscope base.

Optional Accessories

Discusses connecting a camera via adapter and provides a table for magnification calculations.

Ergomodule*

Explains how to use the ergomodule for raising the eye level of the tube opening.

Ergolift*

Describes the base with adjuster wheels for optimizing the working position.

Magnification Changer*

Details the optional magnification changer and its available magnification factors.

Inserting the batteries (DM1000 LED only)*

Guides on how to insert and use rechargeable batteries for the DM1000 LED microscope.

Connection to the Power Supply

Explains how to connect the microscope stand and accessories to the external power supply.

Start-up

Switching on the Microscope

Provides instructions on how to switch on the microscope and initial cautions for gas discharge lamps.

Köhler Illumination*

Details the procedure for setting up Köhler illumination for transmitted light brightfield illumination.

Checking Phase Contrast Rings

Explains how to check the centering of light rings for phase contrast objectives.

Adjusting the Light Sources

Guides on centering the 106z lamp housing and adjusting the arc image for proper illumination.

Operation

Switching on

Instructions for switching on the microscope, particularly with gas discharge lamps.

Stages and Object Displacement

Covers lengthening the coaxial pinion and adjusting the travel range of the stage.

Right-/Left-hand Operation

Details how to change the side of the stage's coaxial pinion for right- or left-hand operation.

Focusing

Explains the operation of coarse and fine focusing wheels and height adjustment of focusing wheels.

Tubes

Discusses adjusting viewing distance, viewing angle, and beam splitting in photo tubes.

Eyepieces

Provides guidance on using eyepieces, including inlaid reticles and vision correction.

Objectives

Covers changing objectives, using immersion oil, and lockable immersion objectives.

Light sources

Details how to adjust brightness for transmitted light and switch on fluorescence lamps.

Aperture Diaphragm

Explains the function of the aperture diaphragm in controlling resolution, depth of field, and contrast.

Field diaphragm

Explains the role of the field diaphragm in protecting the specimen and improving contrast.

Contrast Methods

Transmitted Light

Details transmitted light contrast methods like brightfield, phase contrast, and darkfield.

Brightfield

Outlines the steps for setting up and using brightfield illumination with the microscope.

Phase Contrast*

Guides on setting up and using phase contrast, including objective selection and condenser disk configuration.

Darkfield*

Explains how to set up and use darkfield illumination with specific condensers and objectives.

Oblique Illumination*

Details how to achieve relief-like contrast using oblique illumination with specific condensers.

Polarization*

Describes the procedure for setting up and performing polarization microscopy, including polarizer and analyzer alignment.

Fluorescence*

Provides instructions for setting up and using the fluorescence illumination system.

Measurements with the Microscope

Linear Measurements

Explains the required tools and calibration process for performing linear measurements with the microscope.

Thickness Measurements

Details the method for measuring object thickness using stage height differences and refractive indices.

Object Marker*

Describes the object marker tool used for scribing circles to mark objects during measurements.

Differentiation of Gout / Pseudo Gout

Outlines the procedure for differentiating gout and pseudo gout crystals using the lambda plate compensator.

Trouble shooting

Stand

Lists common problems related to the microscope stand not responding and their possible causes/remedies.

Illumination

Addresses issues with dark or unevenly illuminated images and flickering illumination.

Focus

Discusses problems with bringing the specimen into focus and their potential causes.

Darkfield

Troubleshoots issues with obtaining definite darkfield contrast.

Polarization

Addresses problems related to achieving polarization contrast.

Phase Contrast

Troubleshoots issues where phase contrast is not possible due to specimen or setup issues.

Fluorescence

Addresses problems with dark or weak fluorescence images.

Stage

Solves issues related to the decrease in the stage's travel range over time.

Care of the Microscope

Dust Cover

Provides guidance on using the dust cover to protect the microscope after use.

Cleaning

Details procedures for cleaning coated parts, glass surfaces, and objectives, including cautions.

Handling Acids and Bases

Offers cautions for handling acids and bases to prevent contact with optics and mechanical parts.

Changing Fuses (DM1000)

Explains how to change fuses in the DM1000 stand and provides safety warnings.

Essential Wear and Spare Parts

Retrofitting Components

Equipping the Condenser Disk*

Guides on how to equip the condenser disk with light rings, compensators, or lenses.

Index

EC Declaration of Conformity

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