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General| Microscope Type | Confocal |
|---|---|
| Objective Turret | Motorized |
| Software | LAS X |
| FALCON | Fast Lifetime Contrast |
| Imaging Mode | FLIM, confocal |
| Laser Lines | 470-670 nm |
| Spectral Detection | Yes, spectral detection with freely adjustable bandwidth |
| Detectors | PMT |
| Detector Technology | GaAsP and PMT |
| Scanner Type | Resonant and Galvo |
| Application | Live cell imaging, FRAP, FRET |
| Dimensions | Varies with configuration |
| Weight | Varies with configuration |
Verify the installed stage adapter and replace if necessary, noting specific types for wellplates and dishes.
Instructions for powering on the microscope, laser, and fluorescent lamp using the main unit's buttons.
Start the computer, log in with credentials, and launch the Las X software application.
Choose the appropriate configuration within the Leica Application Suite X, typically 'machine.xlhw'.
Insert the sample after software startup and select the desired laser configuration.
Turn on the Diode 405 laser for DAPI/Hoechst and the white light laser (WLL) if applicable.
Configure excitation/detection by selecting saved schemes or creating new ones using dyes and detectors.
Guidance on using HyD S for bright channels and HyD X for dim channels or FLIM applications.
Instructions for operating the environmental controller, including heating and CO2 settings.
Save data, check next user's needs, and follow protocol A (short-term) or B (long-term) shutdown.
Turn off specific lasers, shut down software, clean objectives, log off, and manage heating/CO2.
Turn off all lasers, shut down software, clean objectives, microscope, and fluorescent lamp.
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