Leica SP8 Upright confocal microscope 14, 07, 2020
• System description:
• DMi6, Upright microscope: BF, Fluorescence (blue, green, red)
• BF TL Detector (transmitted light image)
• Scanning stage with z-Galvo and Navigator (overviews, selection of regions of
interest…)
• Scanner with Scan-field Rotation
• 2 PMT detectors (photo multipliers)
• 1 Hybrid Detector normal (more sensitive than PMT and no detector noise nor
background)
• 1 Hybrid Detector SMD (fast, for FLIM, FALCON)
• Laser lines: 405, White Light Laser (470-670nm, up to 8 laser lines simultaneously)
• FALCON (FAst Lifetme CONtrast) including Pulse Picker for WLL
• LIGHTNING (Deconvolution) for Superresolution and super sensitive imaging
• Phasor plot for direct examination of lifetime data
• Objectives:
• Objective HC PL FLUOTAR 5x/0.15 Dry , WD=12mm (overview) (506224)
• Objective HC FLUOTAR L 16x/0.60 IMM CORR VISIR, WD=2.5mm (Correction collar for
use with 0.0 - 0.17 mm cover glass) + BABB cleared samples (Oil, water, glycerol, silicon
oil)- (506533)
• Objective HC FLUOTAR L 25x/0.95 Water-VISIR, WD=2.5 mm, wide angle (41°) (506374)
• Objective PL APO 20x/0.75 IMM CORR VISIR, WD 0.67 mm (Oil, glycerol)- (506343)
• System Start Up
Turn on heating Unit if needed
• Switch on ‘PC-microscope’ button (1). This gives power to the computer and
microscope controller
• Switch on ‘scanner power’ (2)- This enables the scanning head
• Switch on ‘Laser power’ (3)
• Turn the ‘Laser ignition key’ (4) in position ‘on’- enables Laser shutters (-> Only an
interlock)
• Switch on fluorescent lamp(5) for visual examination
• TL/LED manual switch is at the back of the mic (if you don’t see the TL light in the
eyepiece, it might be the switch)
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