• ATTENTION!!! in line sequential only PMTs and Lasers are switched on/off between
the lines (laser shutters & detector bandwidth remain the same, NO HARDWARE
MOVEMENT between lines is possible !!!)
• Press ‘+’. Set up next configuration, and so on. Set optimal imaging conditions for
each configuration.
TIP-> As an alternative you can optimize your detection/ crosstalk and channels in
line sequential mode and then duplicate the setting (press the +) and deactivate the
lasers that are not needed. This way you can reduce the crosstalk and increase the
speed of imaging.
• You can save "sequential files" in your folder and load them again.
Additional info @:
file:///C:/Program%20Files/Leica%20Microsystems%20CMS%20GmbH/LAS%20X/BIN
/UserHelp/UserHelpEnglish/Default.htm?Highlight=lineseq#INS/INS_sequential_scan
.htm?Highlight=line seq
• Setting up a Z-stack
• Joystick is always set to z-wide, if zgalvo is selected ‘Z position’ knob on the
control panel should be used for setting up the z-stack!!! Changing the
microscope focus knob/joystick will move the whole stage (z-wide)-> therefore
the z-stack will be shifted
• Acquisition mode xyz
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