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Leica TCS SP8 STED 3X User Manual

Leica TCS SP8 STED 3X
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User Manual
Leica TCS SP8 STED 3X
Leica TCS SP8 STED-ONE
From Eye to Insight

Table of Contents

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Leica TCS SP8 STED 3X Specifications

General IconGeneral
Super-resolutionYes
SoftwareLeica Application Suite X (LAS X)
TypeConfocal Microscope
TechnologySTED (Stimulated Emission Depletion)
FluorophoresVarious, compatible with STED
Laser Lines405 nm, 488 nm, 552 nm, 640 nm
DetectorsHybrid detectors (HyD), PMT detectors
ObjectivesVarious objectives available, including oil immersion, water immersion, and dry objectives with different magnifications and numerical apertures (NA)
ScanningGalvo scanner, resonant scanner
Dimensions (W x D x H)Varies depending on configuration; typically a large system requiring significant bench space

Summary

About this User Manual

Intended Use

Liability and Warranty

Important Information for Operators and Users

Key responsibilities and requirements for system operators and users.

Meaning of Warning Messages

General Safety Notes

Commissioning and Use

Guidelines for safe system setup, handling, and initial operation.

Electrical Safety

Precautions for safe electrical connection and operation to prevent shocks.

Safety Devices and Labels

Information on system safety devices and the importance of labels.

Laser Safety

Essential safety measures and precautions for laser operation.

Contact with Liquids

Warnings and precautions regarding exposure to liquids and humidity.

Modifications to the System

Restrictions and guidelines regarding system modifications or component placement.

System Malfunctions

Actions to take in case of specific system malfunctions for safety.

Additional Notes on Handling

Location Requirements

Guidelines for selecting a suitable location for the system.

Using the Software

Best practices and warnings for using the system software.

Protecting the System

Measures to protect the system from damage and ensure longevity.

System Overview and Properties

System Variants

Details on different system configurations available.

Controls on the Supply Unit

Identification and function of controls on the supply unit.

Technical Data

Key technical specifications including dimensions and electrical data.

Electromagnetic Compatibility

Information regarding the system's electromagnetic compatibility.

Serial Number

Location of the system's serial number label.

Ambient Conditions

General Requirements

General requirements for the operating environment.

Vibrations

Guidelines for minimizing vibrations for optimal performance.

Room Dimensions

Recommended room size and space around the system.

Electrical Connection Requirements

Detailed requirements for electrical connections and power supply.

Load Capacity

Information on the load capacity of the supply unit's power outlet.

Waste Heat and Cooling

Considerations for waste heat management and system cooling.

Laser

Laser Classes

Classification of lasers used in the system and associated safety.

Usable Lasers

Overview of visible and infrared lasers compatible with the system.

Safety Features

Laser Safety Chamber

Function and connection of the laser safety chamber.

Main Circuit Breaker

Purpose and location of the main circuit breaker for power disconnection.

Key Switches

Operation of key switches for system and laser access control.

Emission Warning Indicators

Function of indicators signaling laser operational readiness.

Interlock Connectors

Details on interlock connectors for system safety.

Safety Switches on the Microscope

How safety switches on the microscope interrupt laser paths.

Warning Messages

Interpretation of system warning messages and required actions.

Laser Safety Equipment

Description of laser protection tubes and shields.

Safety Labels on the System

Laser Safety Chamber Labels

Safety labels found on the laser safety chamber.

Microscope Safety Labels

Safety labels on inverted and upright microscopes.

Scan Head Labels

Safety labels located on the scan head.

STED Module Labels

Safety labels found on the STED module.

Supply Unit Labels

Safety labels on compact and flexible supply units.

Beam Coupling Unit Labels

Safety labels on the beam coupling unit.

Cover for Replacement Flange Labels

Safety labels on the cover for the replacement flange.

HyD RLD and White Light Laser Labels

Safety labels on HyD RLD and white light laser.

Switching the System On/Off

Flexible Supply Unit On/Off

Step-by-step guide for switching the system on and off.

Compact Supply Unit On/Off

Step-by-step guide for switching the system on and off.

HyD RLD Operation

Instructions for operating HyD reflected light detectors.

LAS X Software

Starting LAS X

Procedure for launching and configuring the LAS X software.

Graphical User Interface

Overview of the LAS X software's graphical interface elements.

STED Laser Control

How to activate and deactivate the STED laser within LAS X.

LAS X Online Help

Accessing and utilizing the LAS X software's online help resources.

Changing the Specimen

Upright Microscope Specimen Change

Steps for changing specimens on an upright microscope.

Inverted Microscope Specimen Change

Steps for changing specimens on an inverted microscope.

Changing the Objective

Lambda/4 Plate Holder

Changing the Filter Cube

Steps for changing filter cubes while using the plate holder.

Care and Cleaning

Cleaning Surfaces

Guidelines for cleaning system surfaces to avoid damage.

Cleaning Optical Components

Recommendations for cleaning optical components safely.

General Care

General advice for maintaining the system's condition.

Repairs and Service Work

Maintenance

Coolant Replacement

Procedure and requirements for coolant replacement.

Cooling Systems on External Devices

Maintenance for cooling systems of external lasers and peripherals.

Refilling External Coolant Tank

Steps for refilling the external coolant tank.

Disassembly and Transport

Disposal

Contact

Appendix

Patents

List of US patents protecting the system.

Safety Data Sheets

Reference to safety data sheets for third-party manufacturers.

Compliance

System compliance with relevant international standards.

People's Republic of China

Administrative measures for electronic information products in China.

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