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Leica TCS SP8 X User Manual

Leica TCS SP8 X
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10
Living up to Life
User Manual
Leica TCS SP8
Leica TCS SP8 MP
Leica TCS SP8 X

Table of Contents

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Leica TCS SP8 X Specifications

General IconGeneral
TypeConfocal Microscope
Laser Lines405 nm, 488 nm, 552 nm, 638 nm
DetectorsHyD (Hybrid Detectors), PMTs (Photomultiplier Tubes)
SoftwareLAS X (Leica Application Suite X)
Objective Lens MountLeica standard
Objective Magnification5x to 100x (depending on the objective used)
DimensionsVaries depending on configuration
WeightVaries depending on configuration
Scanning MethodGalvanometric scanner, resonant scanner (optional)

Summary

About this User Manual

Intended Use

Liability and Warranty

Important Information for Operators and Users

Crucial information for operators and users regarding safety and responsibilities.

Meaning of the warning messages in the manual

General Safety Notes

Commissioning and Use

Guidelines for safe system setup, operation, and handling.

Safety Devices and Safety Labels

Information on safety devices and the importance of safety labels.

Laser Safety

Details laser classification, precautions, and safe handling of laser radiation.

Electrical Safety

Requirements for safe electrical connections, grounding, and shock prevention.

Additional Notes on Handling the System

Location

Recommendations for optimal system placement and environmental conditions.

Using the Software

Instructions for operating the LAS AF software and managing data.

System Overview and Properties

TCS SP8 System Variants

Describes different configurations of the TCS SP8 system.

TCS SP8 MP System Variants

Describes different configurations of the TCS SP8 MP system.

TCS SP8 X System Variants

Describes different configurations of the TCS SP8 X system.

TCS SP8 with external UV laser

Overview and dimensions of the TCS SP8 with an external UV laser.

Controls on the Supply Unit

Details the controls on the flexible and compact supply units.

Technical Data

Provides technical specifications including dimensions and electrical data.

Electromagnetic Compatibility

Information regarding the system's electromagnetic compatibility (EMC).

Serial Number

Location of the system's serial number on the scan head.

Ambient Conditions

General Requirements Regarding Ambient Conditions

Requirements for dust-free indoor areas, lighting, temperature, and humidity.

Vibrations

Guidelines for minimizing vibrations to ensure optimal system performance.

Room Dimensions

Specifies minimum space requirements for different system configurations.

Electrical Connection Requirements

Details electrical connection requirements for various system configurations.

Load capacity of the multiple socket outlet on the flexible supply unit

Maximum load capacity for the flexible supply unit's socket outlet.

Waste Heat and Cooling

Details waste heat output and cooling requirements for various system configurations.

Laser

Laser Classes

Classification of lasers and their wavelength ranges.

Overview of usable lasers

Lists the visible, UV, and IR lasers available for the systems.

IR lasers for TCS SP8 MP

Lists IR lasers for the TCS SP8 MP system.

Safety Features

Main Circuit Breaker for Disconnecting the Power Supply

How to disconnect power using the main circuit breaker.

Key Switch

Details the function and location of key switches for laser control.

Emission Warning Indicators

Explains the function of emission warning indicators.

Interlock Connectors

Details interlock connectors for safety interlocks.

Safety Switches on the Microscope

How safety switches on the microscope interrupt the laser beam path.

Warning Messages

Describes warning messages for defective shutters or audible signals.

Special Laser Safety Equipment

Details special laser safety equipment.

Safety Labels on the System

Inverted microscope

Details safety labels for the inverted microscope.

Upright Microscope

Details safety labels for the upright microscope.

Scan Head

Details safety labels on the scan head.

Compact Supply Unit

Details safety labels on the compact supply unit.

Flexible Supply Unit

Details safety labels on the flexible supply unit.

MP Beam Coupling Unit

Details safety labels on the MP beam coupling unit.

Cover for replacement flange

Details safety labels on the cover for the replacement flange.

Mirror Housing

Details safety labels on the mirror housing.

White light laser

Details safety labels on the white light laser.

External UV Laser

Details safety labels on the external UV laser.

Switching On the System

TCS SP8/SP8 X with flexible supply unit

Switch-on procedure for TCS SP8/SP8 X with flexible supply unit.

TCS SP8 with compact supply unit

Switch-on procedure for TCS SP8 with compact supply unit.

HyD Reflected Light Detectors (HyD RLDs)

Instructions for switching on HyD RLDs before starting LAS AF.

LAS AF

Starting LAS AF

Instructions on how to launch and initialize the LAS AF software.

Structure of the Graphical User Interface

Explains the layout and components of the LAS AF GUI.

LAS AF Online Help

Details on accessing and using the LAS AF online help system.

Changing the Specimen

Changing the Specimen on an Upright Microscope

Procedure for changing specimens on an upright microscope.

Changing the Specimen on an Inverted Microscope

Procedure for changing specimens on an inverted microscope.

Changing the Objective

Piezo Focus on Upright Microscope

Switching Off the System

TCS SP8/SP8 X with flexible supply unit

Switch-off procedure for TCS SP8/SP8 X with flexible supply unit.

TCS SP8 with compact supply unit

Switch-off procedure for TCS SP8 with compact supply unit.

Care and Cleaning

Cleaning Surfaces

Guidelines for cleaning system surfaces using appropriate materials.

Cleaning the Optical System

Instructions for cleaning optical components, including objectives.

Cleaning Immersion Lenses

Procedure for cleaning oil immersion lenses.

Repairs and Service Work

Maintenance

Having Coolant Replaced

Information on replacing system coolant by authorized Leica Service.

Disassembly and Transport

Disposal

Contact

Appendix

Patents

Lists US patents protecting the Leica TCS SP8 and TCS SP8 X products.

Safety Data Sheets from Third-Party Manufacturers

Provides safety data sheets for third-party components.

Compliance

Details the system's compliance with relevant standards.

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