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General| Type | Confocal Microscope |
|---|---|
| Laser Lines | 405 nm, 458 nm, 476 nm, 488 nm, 496 nm, 514 nm, 561 nm, 594 nm, 633 nm |
| Software | Leica Application Suite X (LAS X) |
| Detectors | Hybrid Detectors (HyD), Photomultiplier Tubes (PMT) |
| Objective Lenses | Compatible with Leica HC PL APO objectives (5x to 100x) |
| Scanning Modes | FRAP, FRET, FLIM |
| Application | Live cell imaging, spectral imaging |
| Spectral Detection | Continuous spectral detection with tunable bandwidth |
| Dimensions | Variable, depending on configuration |
| Weight | Variable, depending on configuration |
Instructions for safe system setup, operation, and handling, emphasizing laser safety.
Information on familiarizing with safety devices and the importance of not removing or deactivating safety labels.
Details on laser classes, required safety measures, and precautions for eye and skin protection from laser radiation.
Guidelines for safe electrical connection, grounding, and handling of components to prevent shock.
Measures to protect the system from damage, including maintenance, cleaning, dust, and chemical exposure.
Detailed specifications for power supply, voltage, frequency, and connection requirements for different units.
Safety measures for using APD detectors, including power-on procedures and light exposure.
Description of the main circuit breaker function for power disconnection on compact and flexible supply units.
Location of the detachable key switch for the white light laser for protection against unauthorized use.
Location and voltage of the interlock connector on the white light laser and its use with remote interlocks.
Location of the interlock connector on the scan head and its role in integrating microscope safety switches.
Explanation of safety switches on the microscope and how they interrupt the laser beam path.
Description of laser protection tubes and shields used in inverted microscopes.
Explanation of the safety beam guide and fiber optic cable for VIS, UV, and IR lasers in MP systems.
Information on master key switches for protection against unauthorized laser use and interlock circuits.
Location of the key switch on the power supply of external UV lasers.
Explanation of emission warning indicators that signal laser readiness and potential radiation.
Actions to take in case of emission warning indicator malfunction, including immediate power disconnection.
Details on the use and reinstallation of laser protection tubes and shields for inverted microscopes.
Location and function of the master key switch on the compact supply unit for laser safety.
Description of interlock connectors on the supply unit for remote safety interlocks.
Location and function of the master key switch on the flexible supply unit for laser safety.
Details on the interlock connector on the supply unit and its function in interrupting laser paths.
Explains warning signals for intense light on APDs and the automatic shut-off feature.
Step-by-step instructions for switching on the confocal system with a flexible supply unit.
Step-by-step instructions for switching on the confocal system with a compact supply unit for FLIM.
Instructions for using HyD RLDs, including connection, power-on sequence, and status LED meanings.
Sequence for starting SMD upgrade hardware and software, assuming the confocal system is running.
Instructions for launching the LAS AF software and selecting the operating mode (resonant or conventional).
Explanation of the FLIM Wizard's structure and steps for capturing and optimizing FLIM image data.
Guide to the FCS Wizard's steps for capturing and optimizing image data for FCS measurements.
Procedure to activate lasers as excitation sources within the TCS SP8 LAS AF configuration menu.
Instructions for using MP lasers, including opening shutters and configuring laser parameters.
Instructions for using pulsed UV lasers, including opening shutters and activating the laser line.
Steps for using a pulsed white light laser, including selecting lines and adjusting intensity.
Steps for acquiring images using external APDs, including electrical switch-on and beam path settings.
Instructions for using external FLIM photomultipliers, including connection and LAS AF simulator.
Steps to optimize FLIM measurement conditions by setting instrument parameters in the FLIM Wizard.
Detailed steps for FLIM data acquisition using internal SP FLIM detectors.
Guidance on selecting laser lines for FLIM measurements, emphasizing the use of pulsed lasers.
Guide to setting up time series FLIM measurements at multiple points after optimizing conditions.
Instructions for controlling FLIM measurements, including starting, stopping, and monitoring count rates.
Guidance on selecting laser lines for image acquisition, referencing Chapter 15 for mode of operation.
How to select FLIM detectors (internal, external, non-descanned) for FLIM data acquisition.
Steps for optimizing FLIM settings, including finding specimen position and running test measurements.
Instructions for configuring internal photomultipliers, including beam path settings and activation.
Steps for starting FLIM data acquisition using LAS AF and SymPhoTime.
Importance of the IRF for accurate lifetime measurements and methods for its determination.
Steps for running an IRF measurement, including test mode operation and oscilloscope analysis.
Guide to optimizing the photon count rate for FLIM measurements using test mode.
Steps to set up the imaging process in the FCS Wizard for image acquisition.
Steps helpful in preparing for an FCS measurement, excluding the measurement description itself.
Initiating the FCS Wizard in LAS AF by selecting the FCS operating mode.
Steps to adjust the objective's correction ring for proper detection volume and optimal focal plane.
Guidance on selecting laser lines for image acquisition, referencing Chapter 15 for mode of operation.
Steps to optimize FCS measurement conditions, including selecting APDs and laser lines.
Steps for optimizing FCS settings, including finding measuring points and running test measurements.
Guide to running FCS time series measurements automatically at defined points.
How to define a time series for FCS measurements at multiple points.
Instructions for operating FCS measurement series, including starting, stopping, and monitoring.
Steps for using pulsed VIS lasers for image acquisition, including shutter control and laser activation.
Guidance on selecting detectors (photomultipliers, APDs) for image acquisition in FCS.
Procedure for calibrating and optimizing positioning accuracy for FCS measuring points.
Steps for using continuous wave VIS lasers in FCS, including AOTF slider and beam splitter settings.
Steps to start FCS data acquisition, including starting the Wizard, scanning, and optimizing settings.
Step-by-step instructions for safely switching off the system with a flexible supply unit.
Step-by-step instructions for safely switching off the system with a compact supply unit for FLIM.
Information on authorized service, data backup, and safety precautions during repairs.
Troubleshooting steps for decreased instrument sensitivity, checking laser power and detector alignment.
Troubleshooting steps when no FLIM image is displayed, checking shutters, lasers, and ports.
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