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Leica TCS SP8 User Manual

Leica TCS SP8
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Leica TCS SP8
Quick Start Guide

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Questions and Answers:

Leica TCS SP8 Specifications

General IconGeneral
Microscope TypeConfocal Laser Scanning Microscope
SoftwareLeica Application Suite X (LAS X)
Laser Lines405 nm, 488 nm, 552 nm, 638 nm
DetectorsHybrid Detectors (HyD), Photomultiplier Tubes (PMT)
XY Resolutionup to 120 nm
Scanning SystemGalvanometric scanners
StageMotorized stage
DimensionsVaries depending on configuration
WeightVaries depending on configuration
Objective LensesVarious objectives available, including oil immersion, water immersion, and dry objectives with magnifications from 5x to 100x and numerical apertures from 0.1 to 1.4

Summary

Leica TCS SP8 System Overview

Leica TCS SP8 Scan Head

Start-Up Procedure

Shut-Down Procedure

Turning on the Lasers

Activate Lasers via Beam Path Settings

Click '+' in Laser Lines Box to open Laser Control Window and activate lasers.

Activate Lasers via Configuration Tab

Alternatively, use the Configuration tab and Laser icon to open the Laser Control Window.

Additional Tools in Configuration Tab

Customize USB Control Panel

Assign parameters and sensitivities to control panel knobs and save/load settings.

Objective Specifications

View detailed specifications for the objectives equipped on the microscope.

Dye Database

Explore excitation and emission spectra of fluorochromes in the dye database.

Beam Path Setting: Manual Configuration

Beam Path Setting: Dye Assistant

Beam Path Setting: Load/Save Settings

Beam Path Setting: Sequential Acquisition

Acquisition Parameters

Acquisition Modes

Select Acquisition Mode

Choose between xyz, xzy, xyt, xyzt, and xyλ modes for image acquisition.

Z Stack Acquisition

Configure Z-stack acquisition by setting begin/end positions and step size.

Time Series Acquisition

Additional Acquisition Mode: Tile Scan

Additional Acquisition Mode: Mark & Find

Region-of-Interest (ROI) Scanning

Transmitted Light Imaging (Brightfield/DIC)

Experiment/Project Tree: Saving Images

Spectral (SP) Detection System

Optimizing Pixel Format (Resolution)

Opening the Comparison View

Linking Displays in Comparison View

Closing the Comparison View

Procedure to close display windows and the comparison view.

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