Last Updated on Sep 2021
BEGINNERS SAFETY MANUAL FOR LEICA TCS SP8
CONFOCAL SYSTEM
Produced by Graham Wright, Fiona Chia, Cris Barzaghi, Wang Jing Fang, Shaalini, Melvin
1. Safety Guidelines ........................................................................................................................ 2
1.1 Lasers .................................................................................................................................... 3
1.2 Immersion Oil ........................................................................................................................ 3
1.3 Metal Halide Lamps (MHL) ................................................................................................... 4
2. Training ....................................................................................................................................... 4
2.1 To arrange a training session: ............................................................................................... 4
3. Online Booking and System Access............................................................................................. 5
3.1 Acknowledgements ............................................................................................................... 6
4. Operation Procedures ................................................................................................................. 6
4.1 Switching ON Protocol .......................................................................................................... 7
4.2 Objective Lens ....................................................................................................................... 7
4.3 Software initiation ................................................................................................................ 8
4.4 Turning on the lasers ............................................................................................................ 8
4.5 Using Dye Assistant tab ......................................................................................................... 8
4.6 Setting Laser & Detector configuration .............................................................................. 10
4.7 Using the Acquisition tab .................................................................................................... 11
4.8 Image Adjustments – Setting the Gain and Offset properly............................................... 12
4.9 How to do a Z stack for a 3D Image .................................................................................... 13
4.10 Saving images and reusing settings .................................................................................. 14
4.11 Switching OFF Protocol ..................................................................................................... 15
5. Troubleshooting ........................................................................................................................ 16
5.1 Troubleshooting Appendix .................................................................................................. 18
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