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Leica SP8 - Z-Stack Configuration; Z-Stack Parameters

Leica SP8
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Fig.6
Select ‘Z-galvoor z-Wide in the ‘Z-stack’ menu
Start scanning with ‘live’.
Use ‘Z positionknob on the control panel, select the first slice.
Press begin’ in the Z-stack menu. Move focus up with ‘Z position’, select
the most superficial slice, press ‘End’.
Set up Z-step size according to your needs or activate system optimized
for Nyquist settings according to the objective and pinhole settings used
Additional info @:
file:///C:/Program%20Files/Leica%20Microsystems%20CMS%20GmbH/LAS%20X/BIN/UserHelp/U
serHelpEnglish/Default.htm#DLG/Acquire/DLG_acquire_z_stack.htm

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