• Acquiring confocal images
Select PMT, choosing FLIM Automatically activates the FLIM mode.
Setup all channels as PMT the system automatically choose PMT or
HyD (if the signal is good avoid using the HyD-> manually change it to a
PMT).
Before you start:
Make sure that the Autosave option is switched off in Open Project!!!
CHECK IF THE HyD SMD 1 DETECTOR COOLING CONTROL LAMP IS GREEN. IF IT'S RED
DON'T USE IT, CONTACT BIF TEAM IMMEDEATELY!!!
• Turn on the lasers from the configurations
• Acquisition mode: xyz
• A simple way to plan your confocal imaging is using the dye assistant. Open the
menu and enter your fluorophores. Choose PMT. Then choose the imaging methods
(simultaneous and/or sequential scanning according to your needs considering cross
talk and imaging speed. Press "Apply" ).
Additional info @:
file:///C:/Program%20Files/Leica%20Microsystems%20CMS%20GmbH/LAS%20X/BIN/UserH
elp/UserHelpEnglish/Default.htm#INS/INS_initial_image_acquisition.htm%3FTocPath%3DSt
ep-by-step%2520Instructions%7CImage%2520Acquisition%7C_____5
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