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logos biosystems LUNA FX7 Series User Manual

logos biosystems LUNA FX7 Series
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User Manual
www.logosbio.com
LBSM-SM-ML-LU7-001
VL2005-02

Table of Contents

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logos biosystems LUNA FX7 Series Specifications

General IconGeneral
Brandlogos biosystems
ModelLUNA FX7 Series
CategoryLaboratory Equipment
LanguageEnglish

Summary

Safety Precautions

Instrument Safety Guidelines

General safety guidelines for operating the instrument safely and avoiding hazards.

Operating Conditions and Disposal

Specifies environmental/electrical requirements and guidelines for instrument disposal.

Personal Safety Guidelines

Guidelines for personal safety, including PPE and handling hazardous materials.

Product Introduction

Product Contents

Lists all components included with the LUNA-FX7™™ Automated Cell Counter.

Product Description

LUNA-FX7™™ Overview

Overview of the device's features, capabilities, and components like touchscreen and ports.

Getting Started

Installation and Setup

Step-by-step guide for installing and setting up the LUNA-FX7™™ instrument.

Instrument Startup and Configuration

LUNA-FX7™™ Startup

Instructions on powering on the instrument and navigating the home screen menus.

Screen Saver Settings

Information on the automatic screen backlight turn-off feature and reactivation.

Counting Cells

Sample Preparation and Loading

Preparing cell samples and loading them onto the appropriate slides.

Performing Cell Counts

Inserting slides, viewing images, adjusting focus, and initiating cell counts.

Image Viewing and Adjustment

Image Viewing and Adjustment

Viewing captured images, adjusting light, focus, and zoom for analysis.

Results Interpretation

Results Interpretation

Interpreting count results, navigating chambers, and viewing cell data.

Data Analysis Tools

Data Analysis Tools

Using histograms for cell size analysis and the dilution calculator for concentration.

Saving and Printing Results

Saving and Printing Results

Saving count data, images, and printing reports.

Reviewing Data

Navigating and Viewing Files

Accessing and reviewing saved cell counting results and images from folders.

Image Review and Reanalysis

Viewing captured images, checking protocols, and reanalyzing data.

Managing Previous Counts

Managing Previous Counts

Accessing a list of previous counts and exporting data to USB.

Protocols

Protocol Management

Creating, editing, and selecting custom and default protocols for cell counting.

Protocol Parameters Overview

Understanding the parameters used in different cell counting protocols.

Brightfield Counting Parameters

Brightfield Counting Parameters

Detailed explanation of parameters for brightfield cell counting modes.

Parameter Descriptions

Explanations for Min/Max Search Size, sensitivity, noise reduction, and dilution factor.

Fluorescence Counting Parameters

Fluorescence Counting Parameters

Details on parameters for fluorescence cell counting, including exposure and threshold levels.

Fluorescence Parameter Details

Fluorescence Parameter Details

Adjusting exposure, threshold, and dilution factor for fluorescence measurements.

Bioprocess Feature

Bioprocess Tracking

Overview of tracking multiple bioprocessing activities and charting growth rates.

Recording Bioprocess Data

Steps to record and save bioprocess data, ensuring correct settings.

Reviewing and Exporting Bioprocess Data

Reviewing and Exporting Bioprocess Data

How to review, export, or delete bioprocessing data.

Bioprocess Data Graphing

Viewing charted results for bioprocess data, including cell concentration and viability.

Quality Control

Quality Control Mode and Registration

Using QC mode and registering validation slides for instrument performance monitoring.

Performing Quality Control

Steps to load registered controls and execute the quality control count.

Registering Validation Controls

Registering Validation Controls

Entering control details like name, product key, assay value, and acceptance limits.

Setting Acceptance Limits

Setting Acceptance Limits

Defining upper and lower boundaries for QC graphing.

Executing Quality Control Runs

Running QC tests, inserting slides, and initiating the count.

Analyzing QC Results

Analyzing QC Results

Confirming QC measurements are within acceptance limits and viewing counting data.

Reviewing and Exporting QC Data

Reviewing and Exporting QC Data

Accessing graphical and tabular QC results and exporting data to USB.

Settings

Screen Settings

Adjusting screen display options.

Cell Counting Configuration

Configuring slide format, chamber area, and staining options for cell counts.

Save and Review Configuration

Save and Review Configuration

Configuring quick save, suffix, scale bar, and custom reports for saving and reviewing data.

Network Settings

Network Settings

Connecting the instrument to a network via Ethernet or WiFi.

Server and Date/Time Settings

Server and Date/Time Settings

Configuring server address for CountWire™ and adjusting local date/time.

Instrument Calibration

Instrument Calibration

Performing instrument calibration steps using calibration beads and slides.

Software Updates

Software Updates

Downloading and installing software updates for the instrument.

Data Transfer via Network

FTP Data Transfer

Instructions for using FileZilla FTP Client to transfer data from the instrument.

Maintenance and Troubleshooting

Instrument Maintenance

Procedures for powering on/off and cleaning the instrument safely.

Troubleshooting Common Issues

Solutions for common problems like inaccurate counts, poor images, and connectivity errors.

Troubleshooting Specific Errors

Troubleshooting Specific Errors

Addressing specific errors like dilution factors, USB issues, and software update problems.

Product Specifications

LUNA-FX7™™ Counter Specifications

Detailed physical and technical characteristics of the LUNA-FX7™™ instrument.

LUNA™™ Slides Specifications

LUNA™™ Slides Specifications

Technical details and materials of the various LUNA™™ compatible counting slides.

Ordering Information

Product Catalog

Catalog numbers and quantities for instruments, slides, reagents, software, and accessories.

Purchaser Notification

Limited Use License

Terms and conditions for the research-use-only license agreement.

Instrument Warranty and Service

Details on the limited warranty period, coverage, and out-of-warranty service.

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