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logos biosystems LUNA FX7 User Manual

logos biosystems LUNA FX7
59 pages
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User Manual
www.logosbio.com
LBSM-SM-ML-LU7-001
Rev. 6

Table of Contents

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logos biosystems LUNA FX7 Specifications

General IconGeneral
Dimensions26 cm (W) x 29 cm (D) x 23 cm (H)
Analysis Time35 seconds
Data OutputCSV, PDF
Weight7.5 kg
Counting Speed35 seconds
ConnectivityUSB, Ethernet
Power Supply100-240 VAC, 50/60 Hz

Summary

Disclaimer and Trademarks

Disclaimer

Legal statement regarding document contents and device usage.

Trademarks

Information on product and company trademarks.

Certification Marks

Certification Marks

Symbols indicating compliance with various regulatory standards.

Safety Precautions

Instrument Safety

Guidelines for safe operation and handling of the instrument.

General Safety

General safety practices for using laboratory equipment.

Operating Conditions

Specifies environmental and power requirements for instrument operation.

Instrument Disposal

Instructions for the proper disposal of the instrument.

Instrument Disassembly

Warning against disassembling the instrument.

Personal Safety

Personal protective equipment and safety guidelines for users.

Safety Guidelines

General advice and recommendations for safe instrument use.

Waste Disposal

Procedures for disposing of used consumables and waste.

Product Introduction

Product Contents

Lists the items included in the LUNA-FX7™ package.

Product Description

LUNA-FX7™ Automated Cell Counter

Overview of the automated cell counting device.

LUNA-FX7™ Cell Counting Slides

Description of compatible cell counting slide formats.

Getting Started

Installation

Guide for instrument setup and placement.

Setup

Initial configuration and connection steps.

Counting Cells

Sample Preparation

Procedures for preparing cell samples for counting.

Sample Staining

Methods for staining cells for brightfield and fluorescence counting.

Sample Loading

Procedures for loading cell samples onto slides.

Slide Insertion & Removal

Steps for inserting and removing slides.

Viewing Images

How to view captured cell images on the screen.

Cell Counting

The process of counting cells using the instrument's interface.

Review

Reviewing Data

Accessing and viewing recorded cell counting results.

Review Images

Examining captured cell images from previous counts.

Reanalyze

Re-processing raw images with different parameters.

Previous Counts

Accessing a list of previously performed cell counts.

Protocols

Protocol Selection

Choosing from available pre-set or custom protocols.

Default Protocol

Overview of pre-set protocols for different counting modes.

Creating Protocols

Steps to create new custom counting protocols.

Editing Protocols

Modifying existing counting protocols.

Load Protocol

Applying a saved protocol for cell counting.

Protocol Parameters

Brightfield Cell Counting Parameters

Settings for brightfield counting modes.

Fluorescence Cell Counting Parameters – Cell Lines & Primary Cells

Settings for fluorescence counting of cell lines and primary cells.

Min./Max. search size

Adjusts the range of cell sizes recognized by the algorithm.

Cell detection sensitivity

Controls sensitivity to background signals and weakly stained cells.

Live cell sensitivity

Adjusts detection of live cells based on membrane integrity.

Noise reduction

Manages detection of background noise and weakly stained objects.

Dilution factor

Used to accurately calculate cell concentrations.

Bioprocess Feature

Bioprocess

Overview of tracking bioprocessing activities and charting data.

Creating a Bioprocess Protocol

Steps to create new bioprocess protocols.

Record Bioprocess Data

How to record and save bioprocess data.

Graph

Viewing charted results of bioprocess data.

Quality Control

Quality Control Mode

Using QC to monitor instrument performance with validation slides.

Quality Control

Performing quality checks using validation slides.

Validation Slide Registration

Registering validation slides before use.

Performing Quality Control

Executing QC runs and interpreting results.

Review

Reviewing past Quality Control results.

Settings

LUNA-FX7™ Settings

Accessing and modifying instrument settings.

Screen Settings

Configuring display and screen-related options.

Cell Counting

Adjusting slide format and chamber selection for counting.

Slide Setting and Counting Chamber Area

Options for slide format and chamber selection.

Autofocus Settings

Configuring autofocus behavior for accurate imaging.

Save & Review

Configuring auto-save and report customization.

Network

Connecting the instrument to a network via Ethernet or WiFi.

CountWire

Enabling and configuring the CountWire system.

Date & Power

Setting the instrument's date, time, and power saver.

S/W & Calibration

Managing software updates and instrument calibration.

Data Transfer via Network

Network Sharing

Accessing instrument data from a PC over a network.

Maintenance and Troubleshooting

Maintenance

Procedures for powering on/off and cleaning the instrument.

Troubleshooting

Solutions for common issues like inaccurate cell counts.

Troubleshooting Common Issues

Addressing problems like incorrect counts, slide issues, data transfer, and software errors.

Product Specifications

Physical and Technical Characteristics

Detailed specifications of the LUNA-FX7 instrument.

LUNA™ Slides

Specifications and types of compatible cell counting slides.

Ordering Information

Instruments

Catalog numbers for the LUNA-FX7 instrument.

Slides and Reagents

Catalog numbers for counting slides and stains.

CountWire

Ordering details for the CountWire system.

IQ/OQ

Ordering information for IQ/OQ protocols.

Accessories

Catalog numbers for optional accessories like printers.

Purchaser Notification

Limited Use Label License

Terms and conditions for product use.

Instrument Warranty

Warranty terms and conditions for the instrument.

Out of Warranty Service

Procedures for obtaining service after the warranty period.

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