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Fluorescence Cell
Counting
Parameters – Cell
lines & Primary
cells, Advanced
mode
Parameters for [Fluorescence cell counting-Cell lines & primary cells, Advanced]
The IQOQ-FL-A on the Protocol List is the protocol that is used for IQOQ with the fluorescence
validation slide.
! Important ! The value of total concentration printed on the validation slide label,
using the IQOQ-FL-A may differ from that of using the default protocol because the
label value is determined with the IQOQ-FL-A protocol, which is for the purpose of
IQOQ and Quality Control mode.
Protocol YEAST is an optimized protocol that is used for yeast cell counting.
The exposure for each channel can be adjusted. Increase exposure if the preview image is
dim and only a few cells are visible. Lower exposure if the preview image is too bright and
background noise is high. Determine optimal exposure values empirically.
Search size refers to the approximate cell size that the algorithm recognizes as potential cell
objects. By adjusting Min. and Max. search size, objects sized within the setting value will be
listed as a potential cell candidate.
Clustered objects larger than the Max. search size will not be excluded from the search.
Rather, the algorithm will utilize morphological information to identify individual objects within
the cluster that are within the search size parameters. For the most accurate results, it is
recommended to set the Min./Max. search size window as narrow as possible to encompass
the expected cell size range.
Declumping sensitivity refers to the sensitivity of cell separation from the cluster of cells.
Higher levels of declumping sensitivity will lead to the detection of more cells in a cluster, but
may recognize internal cell structure as separate objects.
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