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logos biosystems LUNA-FX7 Basic User Manual

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User Manual
www.logosbio.com
LBSM-SM-ML-LU7-001
VL2005-02

Table of Contents

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logos biosystems LUNA-FX7 Basic Specifications

General IconGeneral
ModelLUNA-FX7 Basic
Detection MethodFluorescence and Brightfield
Imaging ModesBrightfield, Fluorescence
TypeCell Counter
Fluorescence Channels2
DisplayTouchscreen
ConnectivityUSB, Ethernet
Data OutputCSV
Power Supply100-240 VAC, 50/60 Hz
Dynamic Range1 x 10^4 - 1 x 10^7 cells/mL

Summary

Safety Precautions

Instrument Safety

General safety guidelines for operating the instrument, including electrical and physical hazards.

Operating Conditions and Disposal

Environmental requirements, instrument disposal, and personal safety measures.

Product Introduction

Product Contents

List of components included with the LUNA-FX7™ Automated Cell Counter upon purchase.

Product Description

LUNA-FX7™ Automated Cell Counter Overview

Detailed description of the instrument's features, including front, side, and back views with component labels.

Getting Started

Installation Guide

Instructions for physically installing the LUNA-FX7™ on a stable surface and connecting accessories.

Instrument Startup and Configuration

LUNA-FX7™ Startup and Interface

Initial power-on procedure, home screen overview, and description of counting modes.

Screen Saver Settings

Configuration for automatic screen dimming and reactivation after inactivity.

Counting Cells

Sample Preparation and Loading

Procedures for preparing cell suspensions and loading appropriate volumes onto various slide types.

Slide Insertion and Removal

Step-by-step guide for safely inserting and removing cell counting slides from the instrument.

Image Viewing and Focus Adjustment

Navigating the live view, adjusting light intensity, focus, and zoom for optimal cell visualization.

Performing Cell Counts and Viewing Results

Executing the counting process, including navigation between chambers and fields of view.

Histograms and Data Visualization

Generating and analyzing cell size histograms and gating parameters.

Dilution Calculator and Data Saving Options

Using the dilution calculator and saving count data in various formats.

Data Review and Management

Reviewing Previous Counts

Accessing and viewing historical cell count data and associated images.

Quick Save Functionality

Utilizing the quick save feature for rapid saving of count results with default settings.

Reanalyzing Cell Counts

Re-processing previously acquired cell count data with alternative protocols.

Protocol Management

Protocol Selection and Creation

Managing existing protocols and creating new custom protocols for specific counting applications.

Editing Protocol Parameters

Modifying parameters within existing protocols to optimize counting accuracy.

Brightfield Cell Counting Parameters

Detailed settings for Brightfield cell counting, including size, sensitivity, and noise reduction.

Advanced Brightfield Parameters (Cont.)

Further Brightfield parameters like live cell sensitivity, noise reduction, and dilution factors.

Fluorescence Cell Counting Parameters

Settings for fluorescence cell counting, including exposure levels, thresholds, and dilution factors.

Bioprocess Feature

Bioprocess Tracking and Protocol Setup

Utilizing the bioprocess feature to track cell batches and set up protocols.

Recording Bioprocess Data

Steps for recording and saving bioprocess data for growth analysis.

Bioprocess Data Review and Export

Accessing, charting, and exporting bioprocess data as CSV files.

Quality Control

Quality Control Mode and Validation Slide Registration

Enabling QC mode and registering validation slides for instrument performance checks.

Validation Slide Registration Details

Entering product key, assay value, and acceptance limits for validation slide registration.

Performing Quality Control Runs

Executing a QC run by loading a registered validation slide and initiating counting.

Analyzing QC Results and Re-runs

Confirming QC results against limits and re-running QC if necessary.

Instrument Settings

Screen and Cell Counting Settings

Configuring screen display options, cell counting parameters, and slide formats.

Slide Settings and Autofocus Configuration

Adjusting slide format, chamber selection, staining options, and autofocus behavior.

Save & Review Settings

Customizing quick save options, scale bars, reports, and review preferences.

Network Configuration

Setting up Ethernet or WiFi connections for network access.

Server Settings and Date/Time Configuration

Configuring server addresses for software and adjusting the instrument's date and time.

Instrument Calibration Procedures

Performing calibration steps for the instrument, especially after updates or stain changes.

Software Update Procedures

Procedure for downloading and installing software updates to maintain optimal performance.

Data Transfer via Network

Data Transfer via FTP Freeware

Using FileZilla FTP Client to connect to and transfer data from the LUNA-FX7™.

Maintenance and Troubleshooting

Instrument Maintenance

Guidelines for powering the instrument on/off, cleaning, and touchscreen care.

Troubleshooting Cell Counting Issues

Resolving common problems like clumped cells, incorrect concentrations, and poor image quality.

Troubleshooting Connectivity and Software Issues

Addressing issues with data transfer, USB drives, network connections, and software updates.

Product Specifications

LUNA-FX7™ Automated Cell Counter Specifications

Technical details including storage, cell size range, detection methods, and optics.

LUNA™ Slides

LUNA™ Slides Compatibility and Material

Information on compatible slide types, materials, and physical dimensions.

Ordering Information

Instrument and Accessory Part Numbers

Catalog numbers and quantities for ordering the LUNA-FX7™ instrument and accessories.

Slides, Reagents, Software, and IQ/OQ Ordering

Catalog numbers for slides, reagents, software licenses, and IQ/OQ protocols.

Purchaser Notification

Limited Use Label License

The legal terms governing the use of the product for research purposes.

Instrument Warranty and Service

Details regarding the instrument's warranty period, coverage, and out-of-warranty service.

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