For Total and viability cell counting, prepare a cell suspension according to standard
procedures. Mix the sample, 1:1, with Trypan Blue Stain, 0.4% (T13001) or
Erythrosin B (L13002). Mix gently, but thoroughly to ensure a homogenous
suspension.
For total cell counting, load the sample directly onto the slide without staining the
sample.
Fluorescent cell counting
Prepare a cell suspension according to standard procedures. Mix the sample, 9:1
(cells: stain), with Acridine Orange/Propidium Iodide Cell Viability Kit (F23001). Mix
gently, but thoroughly to ensure a homogenous suspension.
For easy and accurate loading, hold the slides by their edges and pipette at a 45-
60° angle to the slide. Take care not to overload or under-load the chamber.
Press EJECT.
When inserting a slide into the instrument, ensure that the slide is facing up so that
the arrow is showing on the right side and/or so that the lowest chamber designation
is to the left.
Press INSERT. The slide holder will automatically move into position.
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