To turn off the LUNA-FX7
™
, press the power icon in the menu bar or push the power
button for at least three seconds. Turn off the LUNA-FX7
™
at the end of each day.
Turn the LUNA-FX7
™
off and disconnect the power cable before cleaning. Make
sure that liquids do not enter any part of the instrument during cleaning. Do not use
abrasive cloths or bleach solutions as this can cause topical damage.
Surfaces
Clean the surfaces of the instrument with a soft cloth dampened with distilled water.
Wipe dry immediately. Do not pour or spray liquids directly onto the instrument. Do
not wet electrical wires or connections in order to avoid electrical shock or damage.
Touchscreen
Clean the touchscreen with a soft cloth lightly dampened with an authorized LCD
cleansing detergent. Wipe dry immediately. Do not exert excessive force or
pressure as this can damage the touchscreen.
Gently but thoroughly pipette your cell suspension to break up aggregates prior to
counting.
Too few or too many cells
Cell concentrations of 5 x 10
4
to 2 x 10
7
cells/mL are optimal for counting.
Dilute or concentrate cell suspensions accordingly.
Fluorescence signal too strong or weak
Adjust exposure level.
Visible cells uncounted
Adjust the protocol’s detection sensitivity.
Poorly focused images
Make sure the first field is in focus before starting the count. This serves as a
reference for the autofocus function.
Improper slide insertion
Make sure that the slide has been pushed completely to the end of the slide port.
Improper sample loading
Do not over- or under-fill the slide chambers.
Optical components malfunctioning
Optical components may be dirty or damaged.
Please contact your local distributor or Logos Biosystems.
Damaged or contaminated slide
Use a new slide if it is disposable.
Make sure that the counting area of the slide is transparent before loading the
sample.
Wear gloves and handle by the edges to avoid smudging and contamination.
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