29
Navigate to either Brightfield or Fluorescence counting window and press
SETTINGS. Within SETTINGS, software updates, date and time changes, and
background calibrations may be performed. Additionally, options for ‘Cell Counting’,
‘Save & Review’, ‘Network’, ‘Server Setting’ (only with CountWire™ package) and
options may be adjusted.
Choose appropriate slide format and the chamber(s) to be counted.
Slide setting
Select a slide format and Counting chamber area option.
Counting chamber area:
o Current: Counts the chamber that is being viewed in the live view of the
Count screen.
o All: All chambers are counted.
o Chamber designation: One or more chambers may be selected.
When the 3 Ch. slide option is selected, results of all chambers may be combined.
If [Combine results of selected chambers] is ON, the Counting chamber is
automatically set to [all]. When combining results, the PDF report and .CSV file will
contain data from all three chambers.
Staining options
In settings for
This option is used when cell samples are mixed with Logos
Biosystems’ Trypan Blue Stain, 0.4% (T13001) or Erythrosin B
Stain (L13002). Adjust the dilution factor in the protocol as
appropriate.
This option is used when cell samples are mixed with all other
commercial or lab-made vital stains.
Background calibration must be performed when switching
between different brands or compositions.
Autofocused counting
Select On or Off for the LUNA-FX7™ to readjust the focus for each field of view
during image capture (recommended to keep ‘On’.)
Autofocus upon slide insertion
To Next Page
Loading...
Need help?
General
