logos biosystems LUNA-FX7 Basic User Manual

20
Fluorescence cell counting
parameters
Parameters for [Fluorescence cell counting-Cell lines & primary cells]
Parameter
Range
DEFAULT*
GF exposure level
0.1-10
5
RF exposure level
0.1-10
5
Min. cell size
1-89
3
Max. cell size
2-90
70
GF threshold level
1-10
5
RF threshold level
1-10
5
Dilution factor
1-10
1.11
GF/RF exposure level
The exposure for each channel can be adjusted. Increase exposure if the preview
image is dim and only a few cells are visible. Lower exposure if the preview image
is too bright and background noise is high. Determine optimal exposure values
empirically.
Min./ Max. cell size
Use these parameters to adjust the minimum and maximum cell sizes to be included
in results. The base unit is 1 micrometer.
GF/RF threshold level
Green and Red fluorescence threshold will determine the level of threshold during
the image processing. Increasing the threshold will lead to fewer cells being
detected by increasing the background level that is subtracted. Conversely,
decreasing the threshold will lead to more cells being detected.
Dilution factor
The default dilution factor is pre-set as 1.11 for the standard Acridine Orange and
Propidium iodide staining protocol, (e.g. 18 µL cells + 2 µL AO/PI.)
Assuming a final volume of 20 µL, the dilution factor may be adjusted according to
the table below:
Dil. Factor
1
1.11
1.25
1.44
1.66
2
2.5
3.33
5
10
Samp. Vol.
20 µL
18 µL
16 µL
14 µL
12 µL
10 µL
8 µL
6 µL
4 µL
2 µL

logos biosystems LUNA-FX7 Basic Specifications

General

Brand	logos biosystems
Model	LUNA-FX7 Basic
Category	Laboratory Equipment
Language	English