logos biosystems LUNA-FX7 Basic User Manual

19
Parameter
Range
DEFAULT*
Min. search size (μm)
1-89
7
Max. search size (μm)
2- 90
24
Cell detection sensitivity
1-10
5
Live cell sensitivity
1-10
5
Noise reduction
0-9
5
Dilution factor
1-100
2
Min./ Max. search Size
Search size refers to the approximate cell size that the algorithm recognizes as
potential cell objects. By adjusting Min. and Max. search Size, objects sized within
the setting value will be listed as a potential cell candidate.
Clustered objects larger than the Max. search size will not be excluded from the
search. Rather, the algorithm will utilize morphological information to identify
individual objects within the cluster that are within the search size parameters. For
the most accurate results, it is recommended to set the Min/Max search size window
as narrow as possible to encompass the expected cell size range.
Cell detection sensitivity
Cell detection sensitivity refers to the sensitivity of object separation from the
background. A higher Cell detection sensitivity value will increase detection of
signals from weakly stained cells or smaller objects, but can also increase false
positive calls.
Live cell sensitivity
Live cells with intact cell membranes exclude trypan blue and erythrosin B. The dyes
stain the cytoplasm of dead cells with compromised membranes. As a result, object
intensity of unstained live cells is brighter than the stained dead cells. A higher Live
cell sensitivity will decrease the intensity cutoff value and increase the number of
live cells detected.
Live Cell Sensitivity is not available in the protocol of the Total cell counting mode.
Noise reduction
This option allows for the adjustment of background noise during counting. With
more noise reduction, the instrument will not detect weakly stained objects. With
lower noise reduction, the instrument can detect objects with fainter signals.
Dilution factor
The dilution factor is used to calculate cell concentrations accurately. The default
dilution factor is preset as 1 for Total cell counting and as 2 for Total cell & viability
counting (assuming a 1:1 ratio of stain to cell suspension).
This value can be modified according to the dilution of the original sample in
increments of 1 between 1-10; and, increments of 10 between 10 -100. For users
handling highly dense cell cultures. For highly dense cultures, serial dilutions and
several counts with appropriately adjusted dilution factors may be necessary.

logos biosystems LUNA-FX7 Basic Specifications

General

Brand	logos biosystems
Model	LUNA-FX7 Basic
Category	Laboratory Equipment
Language	English